Products

Enzymes for Research, Diagnostic and Industrial Use

Native Bacillus subtilis L-Alanine Dehydrogenase

Cat No.
NATE-0043
Description
L-Alanine dehydrogenase is an A-stereospecific dehydrogenase that catalyzes the reversible deamination of L-alanine to pyruvate and ammonium. It is important for the geneRation of pyruvate during sporulation. L-Alanine dehydrogenase from Bacillus subtilis has a predominately ordered kinetic mechanism in which NAD binds before L-alanine. Subsequently, ammonia, pyruvate, and NADH are released in that specific order. Optimal pH for the amination reaction is 8.8-9.0, whereas it is 10-10.5 for the deamination reaction. The enzyme is inactivated by divalent metal ions and p-chloromercuribenzoate, mercuric ion being most effective. The inactivation may be reversed by L-or D-cysteine.
Abbr
AlaDH, Native (Bacillus subtilis)
Alias
AlaDH
Source
Bacillus subtilis
Applications
L-Alanine dehydrogenase converts L-alanine to pyruvate and ammonium. L-Alanine dehydrogenase from Bacillus subtilis may be used to study enzyme inactivation and protection
Form
Type I, buffered aqueous glycerol solution, Solution in 50% glycerol containing 10 mM potassium phosphate buffer, pH 7.7; Type II, ammonium sulfate suspension, Suspension in 2.4 M (NH4)2SO4 solution, pH 7.0.
Enzyme Commission Number
EC 1.4.1.1
Activity
Type I, ~30 units/mg protein (Lowry); Type II, > 20 units/mg protein (Lowry).
CAS No.
9029-06-5
Unit Definition
One unit will convert 1.0 μmole of L-alanine to pyruvate and NH3 per min at pH 10.0 at 25°C.
Storage
−20°C
Synonyms
L-Alanine Dehydrogenase; Alanine dehydrogenase; EC 1.4.1.1; 9029-06-5; AlaDH; NAD+-linked alanine dehydrogenase; alpha-alanine dehydrogenase; NAD+-dependent alanine dehydrogenase; alanine oxidoreductase; NADH-dependent alanine dehydrogenase
Download Datasheet:


“AlaDH” Total Products Page

Inquiry
Related Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-1801
EC No.EC 1.4.1.1
CAS No.9029-06-5
SourceE. coli
CatalogNATE-0044
EC No.EC 1.4.1.1
CAS No.9029-06-5
SourceE. coli
CatalogEXWM-1442
EC No.EC 1.4.1.1
CAS No.9029-06-5
Source
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-1899
EC No.EC 1.4.1.1
CAS No.9029-06-5
SourceBacillus stearothermophilus
Related Services
Related Protocols
L-alanine_dehydrogenase -Enzymatic Assay Protocol
Online Inquiry
Name:
*Phone:
*E-mail Address:
Country:
Company/Institution:
*Products or Services Interested:
Quantity:
Project Description:
Our Products are Not Intended for Private Therapeutic Use!
*Verification Code:
Please input "enzymes"(case insensitive) as verification code.

Welcome! For price inquiries, please feel free to contact us through the form below through the form on the left side. We will get back to you as soon as possible.

Sitemap  Privacy Policy
Copyright ©2011 - 2017 Creative Enzymes.
Contact Us 45-1 Ramsey Road, Shirley, NY 11967, USA
Email: info@creative-enzymes.com
Tel: 1-631-562-8517 1-631-448-7888
Fax: 1-631-938-8127
Distributors To view the contact information for a specific location, select the desired country: