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Native Bovine Pyruvate Carboxylase

Cat No.
NATE-0508
Description
Pyruvate carboxylase catalyzes the carboxylation of pyruvate to oxaloacetate. Pyruvate carboxylase is a mitochondrial protein that has a biotin prosthetic group that requiries magnesium or manganese and acetyl CoA.
Abbr
PC, Native (Bovine)
Alias
PC
Source
Bovine liver
Species
Bovine
Applications
Pyruvate is critical for gluconeogenesis, lipogenesis, glyceroneogenesis, neurotransmitter biosynthesis and glucose-induced insulin, and is used to study these pr ocesses. The enzyme from Creative Enzymes has been used as a positive control during the assay of pyruvate carboxylase activity in cell-free extracts of Corynebacterium glutamicum.
Form
buffered aqueous glycerol solution; Solution in 50% glycerol containing 0.05 M Tris-HCl, pH 7.4, 2 mM magnesium acetate and 1 mM EDTA.
Enzyme Commission Number
EC 6.4.1.1
Activity
5-25 units/mg protein (BCA)
CAS No.
9014-19-1
Concentration
> 0.5 mg/mL
Unit Definition
One unit will convert 1.0 μmole of pyruvate and CO2 to oxalacetate per min at pH 7.8 at 30°C.
Storage
−20°C
Warnings
Affinity purified
Pathway
Biosynthesis of amino acids, organism-specific biosystem (from KEGG) Biosynthesis of amino acids, conserved biosystem (from KEGG) Biotin transport and metabolism, organism-specific biosystem (from REACTOME) Carbon metabolism, organism-specific biosystem (from KEGG) Carbon metabolism, conserved biosystem (from KEGG) Citrate cycle (TCA cycle), organism-specific biosystem (from KEGG) Citrate cycle (TCA cycle), conserved biosystem (from KEGG)
Function
These data indicate that response of bovine PC gene to thermal stress is through promoter regulation and suggest that there are unique characteristics of bovine PC promoters that may contribute to the physiological response to thermal stress. These data indicate that pyruvate carboxylase promoter 1 is activated by fatty acids found in serum of feed-restricted cows. Expression of pyruvate carboxylase mRNA is part of the adaptive response to feed intake restriction
Synonyms
Pyruvate carboxylase; PC; EC 6.4.1.1; 9014-19-1; pyruvic carboxylase
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Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogEXWM-5812
EC No.EC 6.4.1.1
CAS No.9014-19-1
Source
Realted Protocols
pyruvate_carboxylase -Enzymatic Assay Protocol
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