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Native Canavalia ensiformis (Jack bean) α-Mannosidase


Cat No.
NATE-0754
Description
α-Mannosidase is an acid hydrolase which is located in plant vacuoles and is thought to be involved with the turnover of N-linked glycoproteins. α-Mannosidase has been shown to inhibit the proliferation of B-lymphocytes. α-Mannosidase from Canavalia ensiformis is a tretamer composed of two subunits that each contain two components at 44 and 66 kDa.
Abbr
α-Mannosidase, Native (Canavalia ensiformis)
Source
Canavalia ensiformis (Jack bean)
Applications
Liberates mannose from a variety of synthetic and natural α-mannosides. α-Mannosidase can be used to liberate mannose from a variety of synthetic and natural α-mannosides. It has also been used in a study to investigate the causes of neurodegeneration in mucolipidosis II ′knock-in′ mice.
Form
ammonium sulfate suspension. Suspension in 3.0 M (NH4)2SO4 and 0.1 mM zinc acetate, pH 7.5
Enzyme Commission Number
EC 3.2.1.24
Activity
> 15 units/mg protein (biuret)
CAS No.
9025-42-7
Unit Definition
One unit will hydrolyze 1.0 μmole of p-nitrophenyl α-D-mannoside to p-nitrophenol and D-mannose per min at pH 4.5 at 25°C.
Storage
2-8°C
Synonyms
α-mannosidase; α-D-mannosidase; p-nitrophenyl-α-mannosidase; α-D-mannopyranosidase; 1,2-α-mannosidase; 1,2-α-D-mannosidase; exo-α-mannosidase; EC 3.2.1.24; 9025-42-7; Mannosidase
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Related Protocols
a-mannosidase -Enzymatic Assay Protocol
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