Description
5'-nucleotidase is an enzyme with system name 5'-ribonucleotide phosphohydrolase. This enzyme catalyses the following chemical reaction:a 5'-ribonucleotide + H2O↔ a ribonucleoside + phosphate. This enzyme has a wide specificity for 5'-nucleotides.
Abbr
AMPase, Native (Crotalus atrox venom)
Form
Liquid. In TRIS-HCl, pH 7.4, 125mM sodium chloride, 1mM magnesium chloride and 10% (v/v) glycerol.
Enzyme Commission Number
EC 3.1.3.5
Unit Definition
One U will release 1pmol phosphate per minute from 200µM 5’-AMP, 30°C in a reaction buffer of 10mM Tris-HCl, pH 7.4, 0.2mM MgCl2.
Synonyms
uridine 5'-nucleotidase; 5'-adenylic phosphatase; adenosine 5'-phosphatase; AMP phosphatase; adenosine monophosphatase; 5'-mononucleotidase; AMPase; UMPase; snake venom 5'-nucleotidase; thimidine monophosphate nucleotidase; 5'-AMPase; 5'-AMP nucleotidase; AMP phosphohydrolase; IMP 5'-nucleotidase; EC 3.1.3.5
