Description
Creatine Amidinohydrolase catalyzes the hydrolytic reaction converting creatine to sarcosine and urea. The enzyme is purified from a microorganism. The molecular weight of the enzyme is approximately 67,000. The enzyme is useful for the enzymatic assay of creatine and creatinine when coupled with other related enzymes. creatine + H2O → sarcosine + urea
Abbr
Creatinase, Native (Microorganism)
Applications
This enzyme is useful for enzymatic determination of creatinine when coupled with creatinine amidohydrolase, sarcosine dehydrogenase or sarcosine oxidase and formaldehyde dehydrogenase in clinical analysis.
Appearance
White amorphous powder, lyophilized
Enzyme Commission Number
EC 3.5.3.3
Activity
GradeⅡ 4.0 U/mg-solid or more
Contaminants
NADH oxidase < 5.0×10⁻²%; Catalase < 2.0%
Molecular Weight
approx. 67 kDa (by gel filtration)
Isoelectric point
4.5±0.1
pH Stability
pH 4.0-10.0 (25°C, 20hr)
Michaelis Constant
4.5×10－3 M (Creatine)
Structure
2 subunits per mol of enzyme
Optimum temperature
40－50°C
Thermal stability
below 50°C (pH 7.5, 30min)
Stability
Stable at -20°C for at least one year
Inhibitors
Hg⁺⁺, Cu⁺⁺, Ag⁺, SH reagent (NEM), PCMB
Synonyms
Creatine Amidinohydrolase; Creatinase; EC 3.5.3.3
