Native Streptococcus pneumoniae β(1-4)-Galactosidase

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Cat No.

NATE-0974

Description

The enzyme releases non-reducing terminal β(1-4)-linked galactose from oligosaccharides and glycoproteins. This specificity is only evident at enzyme concentrations < 100mU/ml. At higher concentrations, hydrolysis of β(1-3)-linked galactose occurs.

Abbr

GLB, Native (Streptococcus pneumoniae)

Alias

β-gal; GLB

Source

Streptococcus pneumoniae

Applications

Due to its high selectivity the enzyme is an extremely useful reagent for the identification of non-reducing terminal β(1-4)-linked galactose residues. As such the enzyme has been extensively used for detailed structural analysis in conjunction with broader specificity bovine testes β-galactosidase or Jack bean β-galactosidase.

Form

20 mM Tris-HCl, 25 mM NaCl (pH 7.5)

Molecular Weight

220-247 kD

Unit Definition

One unit is defined as the amount of enzyme required to hydrolyze 1 μmole oNP-β-D-galactopyranoside per min at pH 6.0 and 37°C.

Optimum pH

Buffer

5X concentrated buffer which when diluted gives 50 mM sodium phosphate pH 6.0.

Synonyms

β-galactosidase; beta-gal; β-gal; lactase; β-lactosidase; maxilact; hydrolact; β-D-lactosidase; lactozym; trilactase; β-D-galactanase; oryzatym; sumiklat; β-D-galactoside galactohydrolase

"β-gal" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-1745	Native Bacillus circulans β-Galactosidase	EC 3.2.1.23	9031-110-2	Bacillus circulans	Inquiry
NATE-0295	Native Bovine β-Galactosidase	EC 3.2.1.23	9031-11-2	Bovine testes	Inquiry
DIA-189	Native Escherichia coli β-Galactosidase	EC 3.2.1.23	9031-11-2	Escherichia coli	Inquiry
DIA-220	Native Aspergillus oryzae β-Galactosidase	EC 3.2.1.23	9031-11-2	Aspergillus oryzae	Inquiry

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