Pullulanase from Bacillus subtilis, Recombinant

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Cat No.

NATE-1227

Description

Pullulanase is a lipoprotein generated as a precursor containing a 19-amino acid signal peptide followed by a palmitate-modified cysteine residue. The signal peptide gets cleaved prior to secretion into the extracellular matrix.

Abbr

Pullulanase, Recombinant (Bacillus subtilis)

Source

Bacillus subtilis subsp. subtilis str. 168

Form

Supplied in 3.2 M ammonium sulphate

Enzyme Commission Number

EC 3.2.1.41

Activity

84.66 U/mg

CAS_No

9075-68-7

Molecular Weight

84420.4 Da

Purity

>95 % as judged by SDS-PAGE

Concentration

558.77 U/ml

Unit Definition

One unit is defined as the amount of enzyme required to release 1μmol of D-glucose equivalents per minute from soluble starch (9.04 mg/mL; ACS reagent; solubilised by boiling for 5 min in H2O) in 22.59 mM sodium acetate buffer, pH 5.0, containing 0.452 mg/mL BSA, at 37°C.

Optimum pH

~ 5.0

Optimum temperature

> 37°C

Storage

Store at 4°C (shipped at room temperature)

Preparation Instructions

Agitate vial sufficiently to fully homogenise enzyme precipitate before use.

Synonyms

Pullulanase; EC 3.2.1.41; limit dextrinase (erroneous); amylopectin 6-glucanohydrolase; bacterial debranching enzyme; debranching enzyme; α-dextrin endo-1,6-α-glucosidase; R-enzyme; pullulan α-1,6-glucanohydrolase; 9075-68-7; Pullulanase M2

"Pullulanase" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-0643	Native Klebsiella pneumoniae Pullulanase	EC 3.2.1.41	9075-68-7	Klebsiella pneumoniae	Inquiry
NATE-1448	Pullulanase 13A from Thermotoga maritima, Recombinant	EC 3.2.1.41	9075-68-7	E. coli	Inquiry
BRE-1618	Pullulanase for Saccharification		9075-68-7		Inquiry
SUG-003	Pullulanase (Food Grade)		9075-68-7	Bacillus licheniformis	Inquiry

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