Description
Pullulanase is a lipoprotein generated as a precursor containing a 19-amino acid signal peptide followed by a palmitate-modified cysteine residue. The signal peptide gets cleaved prior to secretion into the extracellular matrix.
Abbr
Pullulanase, Recombinant (Bacillus subtilis)
Source
Bacillus subtilis subsp. subtilis str. 168
Form
Supplied in 3.2 M ammonium sulphate
Enzyme Commission Number
EC 3.2.1.41
Molecular Weight
84420.4 Da
Purity
>95 % as judged by SDS-PAGE
Concentration
558.77 U/ml
Unit Definition
One unit is defined as the amount of enzyme required to release 1μmol of D-glucose equivalents per minute from soluble starch (9.04 mg/mL; ACS reagent; solubilised by boiling for 5 min in H2O) in 22.59 mM sodium acetate buffer, pH 5.0, containing 0.452 mg/mL BSA, at 37°C.
Optimum temperature
> 37°C
Storage
Store at 4°C (shipped at room temperature)
Preparation Instructions
Agitate vial sufficiently to fully homogenise enzyme precipitate before use.
Synonyms
Pullulanase; EC 3.2.1.41; limit dextrinase (erroneous); amylopectin 6-glucanohydrolase; bacterial debranching enzyme; debranching enzyme; α-dextrin endo-1,6-α-glucosidase; R-enzyme; pullulan α-1,6-glucanohydrolase; 9075-68-7; Pullulanase M2
