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Rhamnogalacturonan Lyase from Clostridium thermocellum, Recombinant

Cat No.
NATE-1564
Description
Rhamnogalacturonan Lyase is an endo-1,4-α-rhamnogalacturonan lyase. This enzyme catalyses the following chemical reaction: Endotype eliminative cleavage of L-alpha-rhamnopyranosyl-(1->4)-alpha-D-galactopyranosyluronic acid bonds of rhamnogalacturonan I domains in ramified hairy regions of pectin leaving L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end.
Abbr
Rhamnogalacturonan Lyase, Recombinant (Clostridium thermocellum)
Source
E. coli
Species
Clostridium thermocellum
Form
35 mM NaHepes buffer, pH 7.5, 750 mM NaCl, 200 mM imidazol, 3.5 mM CaCl2, 0.02% sodium azide and 25% (v/v) glycerol
Enzyme Commission Number
EC 4.2.2.-
Molecular Weight
76.1 kDa
Purity
>90% as judged by SDS-PAGE
Concentration
0.5 mg/mL
Specificity
Rhamnogalacturonan
Optimum pH
8.0-10.0
Optimum temperature
65 °C
Storage
This enzyme is shipped at room temperature but should be stored at -20 °C.
Synonyms
endo-1,4-α-rhamnogalacturonan lyase; EC 4.2.2.-
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CatalogNATE-1565
EC No.EC 4.2.2.-
CAS No.
SourceE. coli
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