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Transglutaminase 2 from Rat, Recombinant

Cat No.
NATE-1731
Description
Transglutaminase 2 is based on clone IRBPp993H102D. It is N-terminally fused to a hexahistidine-tag resulting in the encoded N-terminal amino acid sequence MHHHHHHAEELNL. Transglutaminase 2 is produced in E. coli and purified by ion metal chelating chromatography to more than 95 % purity.
Abbr
TG 2, Recombinant (Rat)
Alias
TG2
Source
E. coli
Species
Rat
Applications
The transglutaminase 2 catalyzes acyl transfer reactions from glutamine residues in proteins or peptides to primary amines, e. g. the formation of ε-(γ-glutamyl) lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue. The transglutaminase 2 may also be used for immunoprecipitation.
Appearance
White lyophilized solid.
Package
250 μg; 1mg
Form
The transglutaminase is lyophilized from 50 mM NaH2PO4, 150 mM NaCl, pH 8. Sample contains maltodextrin.
Enzyme Commission Number
EC 2.3.2.13
Activity
> 750 U/mg [Activity is determined by measuring the rate of fluorescence enhancement after transglutaminase-catalyzed monodansylcadaverine-incorporation into N,N-dimethylated casein according to Lorand et al., Anal. Biochem. 44 (271-231).
CAS No.
80146-85-6
Molecular Weight
77 kDa
Purity
> 95 % (visually by SDS-PAGE)
Unit Definition
1 U is defined as the increase in fluorescence intensity of 1 a.u./min (measured on a Cary eclipse fluorescence spectrophotometer, Varian; λex = 332 nm, λem = 500 nm; band filter = 5 nm; detector strength = 600 V; temperature = 37°C, assay volume = 1 ml)].
Activators
Add 10 mM Ca2+ to activate transglutaminase.
Storage
Store working aliquots at ≤ - 20°C. Avoid repeated freezing and thawing.
Delivery is possible at ambient temperature.
Synonyms
transglutaminase; EC 2.3.2.13; 80146-85-6; transglutaminase; Factor XIIIa; fibrinoligase; fibrin stabilizing factor; glutaminylpeptide γ-glutamyltransferase; polyamine transglutaminase; tissue transglutaminase; R-glutaminyl-peptide:amine γ-glutamyl transferase; protein-glutamine γ-glutamyltransferase; TG1
Reconstitution
Add the volume of H2O the protein is lyophilized from to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution the solution should be stored frozen in working aliquots. Keep cooled on ice for short term storage.
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Realted Protocols
transglutaminase -Enzymatic Assay Protocol
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