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β-Mannosidase from Bacteroides thetaiotaomicron, Recombinant


Cat No.
NATE-1186
Description
Beta-mannosidase is an enzyme with system name beta-D-mannoside mannohydrolase. This enzyme catalyses the following chemical reaction:Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides. This gene encodes a member of the glycosyl hydrolase 2 family. The encoded protein localizes to the lysosome where it is the final exoglycosidase in the pathway for N-linked glycoprotein oligosaccharide catabolism. Mutations in this gene are associated with beta-mannosidosis, a lysosomal storage disease that has a wide spectrum of neurological involvement.
Abbr
β-Mannosidase, Recombinant (Bacteroides thetaiotaomicron)
Source
Bacteroides thetaiotaomicron VPI-5482
Form
Supplied in 3.2 M ammonium sulphate
Enzyme Commission Number
EC 3.2.1.25
Activity
10.62 U/mg
CAS No.
9025-43-8
Molecular Weight
96040.16 Da
Purity
> 95 % as judged by SDS-PAGE
Concentration
45.27 U/ml
Unit Definition
One unit is defined as the amount of enzyme required to release 1μmol of D-mannose per minute from ivory nut mannan (5 mg/mL; freshly treated with 10 % sodium hydroxide and neutralised with acetic acid) in 50 mM sodium phosphate buffer, pH 5.6, containing 1mg/mL BSA, at 37°C, and using the method of Miller (1957) to follow reducing sugar liberated at 575 nm.
Optimum pH
5.6
Storage
Store at 4°C (shipped at room temperature)
Synonyms
β-mannosidase; mannanase; mannase; β-D-mannosidase; β-mannoside mannohydrolase; exo-β-D-mannanase; EC 3.2.1.25; 9025-43-8
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Related Protocols
b-mannosidase -Enzymatic Assay Protocol
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