Products

Enzymes for Research, Diagnostic and Industrial Use

β-acetylglucosaminidase 73A from Clostridium perfringens, Recombinant

Cat No.
NATE-1289
Description
This enzyme releases non-reducing terminal β1-2, β1-3, β1-4 and β1-6 linked N-acetylglucosamine from complex carbohydrates. When incubated with oligosaccharides at low concentrations (<50 mU/ml) the enzyme can differentiate between GlcNAcβ1-2Man, GlcNAcβ1-4Man and GlcNAcβ1-6Man linkages. Under such conditions, the enzyme cleaves essentially only β1-2 linked GlcNAc, with two provisos. Firstly, β1-2 GlcNAc is not hydrolyzed if the mannose to which it is substituted has a substitution at C-6. Thus, the enzyme is useful for the analysis of tri-antennary oligosaccharides. Secondly, if the β-linked mannose of the conserved pentasaccharide core is substituted with a "bisecting" GlcNAc then only the β1-2 linked GlcNAc linked to mannose on the α1-3 arm is cleaved. At higher concentrations of the enzyme, β1-4 and β1-6 linked GlcNAc may also be hydrolyzed.
Abbr
β-acetylglucosaminidase 73A, Recombinant (Clostridium perfringens)
Source
E. coli
Species
Clostridium perfringens
Form
35 mM NaHepes buffer, pH 7.5, 750 mM NaCl, 200 mM imidazol, 3.5 mM CaCl2, 0.02% sodium azide and 25% (v/v) glycerol
Molecular Weight
24.6 kDa
Purity
>90% by SDS-PAGE
Concentration
0.25 mg/mL
Specificity
Peptidoglycan
Optimum pH
8
Optimum temperature
37 °C
Storage
This enzyme is shipped at room temperature but should be stored at -20 °C.
Synonyms
beta-N-acetyl-D-hexosaminide; N-acetylhexosaminohydrolase; β-N-Acetylhexosaminidase; N-Acetyl-β-D-glucosaminidase, β-N-Acetylglucosaminidase
Download Datasheet:


“β-N-Acetylhexosaminidase” Total Products Page

Inquiry
Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-0779
EC No.EC 3.2.1.52
CAS No.9012-33-3
SourceBovine kidney
CatalogNATE-0780
EC No.EC 3.2.1.52
CAS No.9012-33-3
SourceCanavalia ensiformis
CatalogNATE-0781
EC No.EC 3.2.1.52
CAS No.9012-33-3
SourceHuman placenta
CatalogNATE-0782
EC No.3.2.1.52
CAS No.9012-33-3
SourceE. coli
+ See More >>
Online Inquiry
Name:
*Phone:
*E-mail Address:
Country:
Company/Institution:
*Products or Services Interested:
Quantity:
Project Description:
Our Products are Not Intended for Private Therapeutic Use!
*Verification Code:
Please input "enzymes"(case insensitive) as verification code.

Welcome! For price inquiries, please feel free to contact us through the form below through the form on the left side. We will get back to you as soon as possible.

Sitemap  Privacy Policy
Copyright ©2011 - 2017 Creative Enzymes.
Contact Us 45-1 Ramsey Road, Shirley, NY 11967, USA
Email: info@creative-enzymes.com
Tel: 1-631-562-8517 1-631-448-7888
Fax: 1-631-938-8127
Distributors To view the contact information for a specific location, select the desired country: