Description
Alkaline phosphatase (ALP, ALKP, ALPase, Alk Phos) (EC 3.1.3.1) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. As the name suggests, alkaline phosphatases are most effective in an alkaline environment. It is sometimes used synonymously as basic phosphatase.
Abbr
ALP, Recombinant (Human)
Enzyme Commission Number
EC 3.1.3.1
Activity
> 2,500 units/mg
Molecular Weight
This protein is fused with 6x His tag at C terminus (23-506 aa) and the protein has a calculated MW of 53.9 kDa (118aa). The protein migrates at 50-70KDa in SDS-PAGE under reducing conditions.
Unit Definition
One unit is defined as the amount of enzyme that hydrolyzes 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.
Storage
Store at +4°C for short term (1-2 weeks). For long term storage, aliquot and store at -70°C. Avoid repeated freeze/thaw cycles.
Synonyms
ALPP; Alkaline phosphatase Regan isozyme; Placental alkaline phosphatase 1; PLAP-1
Endotoxin Level
< 1.0 EU per 1μg of protein
