Description
Catalase is an enzyme that catalyzes the transformation of hydrogen peroxide into water and oxygen. This enzyme functions as a natural antioxidant protecting the cell against oxidative damage. This enzyme finds applications in Research and Clinical Chemistry. It also finds diverse industrial applications in textiles, waste treatment, cosmetics and as a disinfectant agent.
Abbr
CAT, Recombinant (Microbial)
Species
Psychrotolerant Bacteria
Enzyme Commission Number
EC 1.11.1.6
Activity
> 8000 U/mg protein
Molecular Weight
~220 kDa
Concentration
Protein concentration: >40% (w/w)
Unit Definition
One unit is defined as the decomposition of 1umol of H2O22 in 1 Unit Definition minute at 25°C at pH 7.0. The rate of disappearance of H2O2 is observed at 240 nm.
Optimum temperature
30-40°C
Thermal stability
20-80°C, Keeps 50% of its activity after 7 hours of exposure at 50°C.
Storage
At -20°C, it keeps 100% of its activity for more than two years
Buffer
0.05M Tris HCl pH 8; 0.5M NaCl and glycerol at 20%
Synonyms
EC 1.11.1.6; Catalase; equilase; caperase; optidase; catalase-peroxidase; CAT; H2O2:H2O2 oxidoreductase
