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Glucokinase 2 from Recombinant E.coli

Cat No.
NATE-1939
Description
Glucose is phosphorylated to glucose-6-phosphate by glucokinases. This gene is alternatively spliced to generate three different forms of the enzyme; one found in the pancreas and two found in the liver. The main function of this gene is to regulate carbohydrate metabolism. Recombinant human pancreatic Glucokinase has a C-terminal FLAG tag and has 470 amino acid residues. It can be useful for studies including enzyme kinetics, activator screening and kinase selectivity.
Source
E. coli
Form
Lyophilized
Enzyme Commission Number
EC 2.7.1.2
Activity
>350 U/mg protein
Contaminants
(as GlcK2 activity = 100 %)
Glucose-6-phosphate dehydrogenase < 0.01 %
Phosphoglucomutase < 0.01 %
6-Phosphogluconate dehydrogenase < 0.01 %
Hexose-6-phosphate isomerase < 0.01 %
Glutathione reductase < 0.01 %
Molecular Weight
ca. 32,000
pH Stability
7.0 - 10.0
Michaelis Constant
(60mM Tris-HCI buffer, pH 8.5, at 30 °C)
Glucose 0.1 mM
ATP 0.05 mM
Unit Definition
One unit of activity is defined as the amount of GlcK2 that forms 1 μmol of glucose 6-phosphate per minute at 30 °C.
Optimum pH
9
Optimum temperature
70 °C
Thermal stability
No detectable decrease in activity up to 60 °C.
Storage
Stable at -20°C for at least one year
Synonyms
EC 2.7.1.2; glucokinase; glucokinase (phosphorylating); 9001-36-9; GCK; FGQTL3; GK; GLK; HHF3; HK4; HKIV; HXKP; LGLK; MODY2; Human pancreatic glucokinase; pancreatic glucokinase
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Related Services
Related Protocols
glucokinase -Enzymatic Assay Protocol
nad-kinase -Enzymatic Assay Protocol
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