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Glucose Dehydrogenase from E. coli, Recombiant


Cat No.
NATE-1902
Description
In enzymology, a glucose 1-dehydrogenase (EC 1.1.1.47) is an enzyme that catalyzes the chemical reaction:beta-D-glucose + NAD (P)+↔ D-glucono-1,5-lactone + NAD (P)H + H+. The 3 substrates of this enzyme are beta-D-glucose, NAD+, and NADP+, whereas its 4 products are D-glucono-1,5-lactone, NADH, NADPH, and H+. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor.
Abbr
GDH, Recombiant (E. coli)
Alias
GDH
Source
E. coli
Applications
This enzyme is useful for determination of glucose.
Appearance
Lyophilized
Enzyme Commission Number
EC 1.1.1.47
CAS No.
9028-53-9
Contaminants
as GlcDH2 activity = 100 %)
NADH oxidase: <0.01 %
Molecular Weight
ca. 126,000; Subunit molecular weight : ca. 31,500.
pH Stability
5.0 - 10.0 (with 3M NaCl)
Michaelis Constant
D-Glucose: 3.7 mM;
NAD+: 0.06 mM;
NADP+: 0.02 mM.
Specificity
D-Glucose: 100 %;
D-Maltose: 1.1 %;
D-Galactose: 0.1 %;
D-Xylose: 3.0 %;
D-Fructose: 0.3 %;
D-Mannose: 4.8 %;
D-Arabinose: 0 %;
Trehalose: 0 %;
D-Lactose: 1.3 %;
D-Sucrose: 0 %;
2-Deoxy-D-Glucose: 100 %;
D-Glucose-1-Phosphate: 0 %;
D-Glucose-6-Phosphate: 0 %;
D-Sorbitol: 0 %;
Unit Definition
One unit of activity is defined as the amount of GlcDH2 that forms 1 μmol of NADH per minute at 37 °C.
Optimum pH
8.5
Thermal stability
No significant decrease in activity up to 70 °C. (with 3M NaCl and 0.1% BSA).
Storage
Stable at -20 °C for at least one year.
Synonyms
EC 1.1.1.47; D-glucose dehydrogenase (NAD (P)+); hexose phosphate dehydrogenase; β-D-glucose:NAD (P)+ 1-oxidoreductase; glucose 1-dehydrogenase; Glucose dehydrogenase; 9028-53-9
Specific Activity
more than 900 U/mg protein
Reaction
D-Glucose + NAD(P)+ ←→ D-Glucono-δ-lactone + NAD(P)H + H+
Download Datasheet:
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“Glucose Dehyrogenase” Total Products Page

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Related Services
Related Protocols
GLUCOSE DEHYDROGENASE -Enzymatic Assay Protocol
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