Description
Hyaluronate lyase degrades hyaluronate using an elimination reaction to break glycosidic linkages yielding unsaturated disaccharide products.
Abbr
Hyaluronate Lyase, Recombinant (Streptomyces coelicolor)
Source
Streptomyces coelicolor A3(2)
Form
Supplied in 3.2 M ammonium sulphate
Enzyme Commission Number
EC 4.2.2.1
Molecular Weight
82914.2 Da
Purity
> 95 % as judged by SDS-PAGE
Unit Definition
One unit is defined as the amount of enzyme required to release 1μmol of 4,5-unsaturated product per minute from 1 mg/mL hyaluronic acid in 50 mM sodium acetate buffer, pH 6.0, at 37°C, as measured at 232 nm.
Storage
Store at 4°C (shipped at room temperature)
Preparation Instructions
Agitate vial sufficiently to fully homogenise enzyme precipitate before use.
Synonyms
hyaluronidase; glucuronoglycosaminoglycan lyase; spreading factor; mucinase; 37259-53-3; EC 4.2.2.1; hyaluronate lyase
