Description
BcKsh111A (GH111), E.C. number 3.2.1.-, is an endo-β -N-acetylglucosaminidase from Bacillus circulans. Recombinant BcKsh111A (GH111), purified from Escherichia coli, is a single domain family 111 Glycoside Hydrolase (GH111).
Species
Bacillus circulans
Form
35 mM NaHepes buffer, pH 7.5, 750 mM NaCl, 200 mM imidazol, 3.5 mM CaCl2, 0.02% sodium azide and 25% (v/v) glycerol
Enzyme Commission Number
EC 3.2.1.-
Molecular Weight
69,07 kDa
Purity
>90% as judged by SDS-PAGE
Specificity
BcKsh111A (GH111) hydrolyses cartilage keratan sulfate and cornea keratan sulfate.
Optimum temperature
55 °C
Storage
This enzyme is shipped at room temperature but should be stored at -20 °C.
Synonyms
endo-β -N-acetylglucosaminidase; single domain family 111 Glycoside Hydrolase; Keratan sulfate Endo-N-acetylglucosaminidase 111A; Keratan sulfate Endo-N-acetylglucosaminidase; GH111
