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Modified T7 RNA polymerase

Cat No.
NATE-1698
Description
A modified T7 RNA polymerase that orthogonally recognizes a non-natural promoter sequence: 5’-TAATACCGGTCACTATA, where the underlined residues differ from wild-type.
Abbr
Modified T7 RNA polymerase, Recombinant (E. coli)
Form
Liquid
Enzyme Commission Number
EC 2.7.7.6
CAS No.
9014-24-8
Molecular Weight
100.142 kDa
Purity
> 98% (SDS-PAGE)
Concentration
0.5mg/Ml
Unit Definition
One unit is defined as the amount of enzyme that will incorporate 1 nmol ATP into acid-insoluble material in a total reaction volume of 50 ul in 1 hour at 37°C in 1X RNA Polymerase Reaction Buffer.
Storage
at -20 °C
Buffer
50 mM Tris, 50 mM NaCl, 1 mM DTT, 0.1% Triton-X 100, 0.1 mM EDTA, 50% glycerol
Synonyms
CGG-R12-KIR
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Related Protocols
ribonucleic acid polymerase -Enzymatic Assay Protocol
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