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Mutant Isocitrate Dehydrogenase 1 R132H from Human, Recombinant

Cat No.
NATE-1649
Description
Mutations of the cytosolic IDH 1 are a common feature in primary human brain cancers. Arginine 132 (R132) of IDH is highly conserved among different isoforms of IDH and is most commonly mutated to Histidine. Mutation of IDH1 R132H leads to accumulation of R(-)-2-hydroxyglutarate (2HG), which correlates with an increased risk for malignant brain tumors.
Abbr
IDH 1 R132H, Recombinant (Human)
Source
E. coli
Species
Human
Product Overview
Full-length human IDH1 (452 amino acids) with N-terminal HATtag. Arginine 132 is mutated to histidine
Form
Lyophilized powder with additives
Activity
>500 mU/mg
Molecular Weight
50.9 kDa
Purity
> 90% by SDS-PAGE
Unit Definition
One unit is the amount of enzyme that will convert 1.0 µmole of NADPH to NADP+ per minute at pH 7.4 at 25°C.
Storage
Lyophilized protein is stable for 1 year at -20°C. Once reconstituted aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Synonyms
Isocitrate dehydrogenase [NADP] cytoplasmic mutant, R132H; IDH 1 R132H
Reconstitution
Resuspend lyophilized protein in 30% glycerol glycerol/water at 2 mg/ml and then further diluted in future applications.
Formulation
In 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 10% glycerol.
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