Description
Endo-β-Galactosidase is an enzyme that hydrolyzes internal β-galactosidic linkages of oligosaccharides in poly-N-acetyl-lactosamine structures. This enzyme resembles the Escherichia freundii enzyme due to its specificity towards bovine corneal keratan sulphate, milk oligosaccharides and the glycolipids lacto-N-neotetraosylceramide and lacto-N-tetraosylceramide.
Abbr
N-Acetylglucosamine endo-β-galactosidase 16C, Recombinant (Clostridium perfringens)
Species
Clostridium perfringens
Form
35 mM NaHepes buffer, pH 7.5, 750 mM NaCl, 200 mM imidazol, 3.5 mM CaCl2, 0.02% sodium azide and 25% (v/v) glycerol
Enzyme Commission Number
EC 3.2.1.-
Molecular Weight
33.7 kDa
Specificity
GlcNAc-α-1,4-Gal-β-1,3-GalNAc-α1-Ser/Thr
Optimum temperature
37 °C
Storage
This enzyme is shipped at room temperature but should be stored at -20 °C.
Synonyms
β-Galactosidase bacterial; Keratanase; Endo-β-galactosidase; keratan sulfate endogalactosidase; keratan-sulfate 1,4-β-D-galactanohydrolase; EC 3.2.1.103
