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Native Bacillus fastidiosus Uricase


Cat No.
DIA-173
Description
The enzyme urate oxidase (UO), or uricase or factor-independent urate hydroxylase, absent in humans, catalyzes the oxidation of uric acid to 5-hydroxyisourate: Uric acid + O2 + H2O → 5-hydroxyisourate + H2O2 → allantoin + CO2
Abbr
UO (Bacillus fastidiosus)
Alias
UO; uricase
Source
Bacillus fastidiosus
Appearance
White to off-white powder
Product Overview
Urate:oxygen oxidoreductase produced in microorganism has a molecular mass of approximately 34 kDa.
Form
Freeze dried powder
Enzyme Commission Number
EC 1.7.3.3
Activity
15 U/mg
CAS No.
9002-12-4
Contaminants
Cholesterol oxidase < 0.005%; Catalase < 1%; Glucose oxidase < 0.005%
pH Stability
5.5-10.0 (25°C for 20 hrs)
Unit Definition
One unit will oxidize one micromole of uric acid at pH8.5 at 25°C.
Optimum pH
7
Optimum temperature
37°C
Thermal stability
Stable at 50°C and below (pH 7.0, 15 mins)
Storage
-20°C
Inhibitors
Ag+, Hg2+
Synonyms
urate oxidase; uric acid oxidase; uricase; uricase; urate: oxygen oxidoreductase; EC 1.7.3.3; uricase II; UO
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Related Services
Related Protocols
URICASE -Enzymatic Assay Protocol
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