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Native E. coli 1-5-Anhydroglucitol-6-Phosphate Dehydrogenase

Cat No.
NATE-0039
Abbr
AG6PDHII, Native (E. coli)
Alias
AG6PDHII
Source
Escherichia coli
Applications
Useful for enzymatic determination of 1,5-AG
Appearance
White powder
Product Overview
AG6PDHII is an enzyme produced by microorganisms. This product shall be used for a diagnostics reagent.
Form
Freeze dried powder
Enzyme Commission Number
EC 1.1.1.140
Activity
> 20 U/mg
CAS No.
37250-69-4
Contaminants
ATPase < 0.05%; NADPH oxidase < 0.02%
Molecular Weight
78 kDa (TSK gel G 3000 SWXL gel filtration); 40 kDa (SDS‒PAGE)
Isoelectric point
pH 4.7
pH Stability
7.0-9.0 (50°C, 30 mins)
Michaelis Constant
1,5‒Anhydroglucitol‒6‒phosphate 25 mM (pH 10.0); NADP 0.09 mM (pH 10.0)
Unit Definition
One unit is defined as the amount of enzyme which converts 1 μmole of 1,5‒AG‒6‒phosphate to C6H11O8P per minute at 37°C under the conditions specified in the assay procedure.
Optimum pH
9.0-10.0
Optimum temperature
37‒50°C
Thermal stability
Stable at 42°C and below (pH 9.5, 30 mins)
Stability
Stable for 1 years under the freezing condition (-80°C).
Storage
Storage at-80°C in the presence of a desiccant is recommended.
Synonyms
AG6PDHII; EC 1.1.1.140; 1-5-Anhydroglucitol-6-Phosphate Dehydrogenase
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EC No.EC 1.1.1.140
CAS No.37250-69-4
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