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Native Microbial Xanthine Oxidase


Cat No.
NATE-0733
Description
Xanthine oxidase is a molybdenum-containing enzyme that is found in the cytosol, and may be strongly inhibited by flavonoids. It plays a vital role in the metabolism of some drugs, as well as purines and pyrimidines. It is also known to be a biological source of reactive oxygen species. Xanthine oxidase was shown to be involved in the reduction of cytochrome c by the generation of superoxide anions following the oxidation of xanthine. These free radicals are responsible for reducing cytochrome c.
Abbr
XO, Native (Microbial)
Alias
XO; XOD
Source
Microbial
Applications
This enzyme is useful for enzymatic determination of inorganic phosphorus, 5′-nucleotidase and adenosine deaminase when coupled with Purine-nucleoside phosphorylase and uricase.
Product Overview
Allopurinol is a synthetic drug show to inhibit xanthine oxidase.
Form
Lyophilized powder containing BSA and sodium glutamate as stabilizers
Enzyme Commission Number
EC 1.17.3.2.
Activity
> 7 units/mg solid
CAS No.
9002-17-9
Molecular Weight
mol wt ~160 kDa
pH Stability
pH 6.5-9.0 (25°C, 15hr)
Unit Definition
One unit will convert 1.0 μmole of xanthine to uric acid per min at pH 7.5 at 25°C. Approx. 50% of the activity is obtained with hypoxanthine as substrate.
Optimum pH
7.5-8.0
Optimum temperature
65°C
Thermal stability
below 55°C (pH 8.0, 30min)
Storage
−20°C
Inhibitors
Reducing agents, Hg++, Ag+,MIA
Synonyms
Xanthine oxidase; XO; xanthine oxidoreductase; EC 1.17.3.2; 9002-17-9; XOD; Xanthine:oxygen oxidoreductase; hypoxanthine oxidase; hypoxanthine:oxygen oxidoreductase; Schardinger enzyme; hypoxanthine-xanthine oxidase; xanthine:O2 oxidoreductase; xanthine:xanthine oxidase
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Catalog
Product Name
EC No.
CAS No.
Source
Price
CatalogDIA-218
EC No.EC 1.1.3.22
CAS No.9054-84-6
SourceMicroorganism
CatalogNATE-0732
EC No.EC 1.17.3.2.
CAS No.9002-17-9
SourceBovine milk
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