Description
In enzymology, a quinoprotein glucose dehydrogenase (EC 1.1.5.2) is an enzyme that catalyzes the chemical reaction: D-glucose + ubiquinone ↔D-glucono-1,5-lactone + ubiquinol. Thus, the two substrates of this enzyme are D-glucose and ubiquinone, whereas its two products are D-glucono-1,5-lactone and ubiquinol. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with a quinone or similar compound as acceptor. This enzyme participates in pentose phosphate pathway. It employs one cofactor, PQQ.
Abbr
Glucose Dehydrogenase, Native (Microorganism)
Applications
This enzyme is useful for enzymatic determination of D-Glucose.
Appearance
Purple amorphous powder, lyophilized
Enzyme Commission Number
EC 1.1.5.2
Activity
GradeⅢ 500 U/mg-solid or more
Contaminants
Glucose dehydrogenase < 1.0×10⁻³% (NAD-dependent); Hexokinase < 1.0×10⁻³%
Molecular Weight
approx. 100 kDa (by gel filtration)
pH Stability
pH 3.5-8.5 (25°C, 16hr)
Michaelis Constant
4.8 mM (D-Glucose)
Thermal stability
below 50°C (pH 7.5, 30min)
Inhibitors
Cu⁺⁺, Pb⁺⁺, Ag⁺
Synonyms
Glucose Dehydrogenase; EC 1.1.5.2; D-glucose:ubiquinone oxidoreductase; D-glucose:(pyrroloquinoline-quinone) 1-oxidoreductase; glucose dehydrogenase (PQQ-dependent); glucose dehydrogenase (pyrroloquinoline-quinone); quinoprotein D-glucose dehydrogenase
