Description
In enzymology, a pyruvate oxidase (EC 1.2.3.3) is an enzyme that catalyzes the chemical reaction: pyruvate + phosphate + O2 ↔ acetyl phosphate + CO2 + H2O2. The 3 substrates of this enzyme are pyruvate, phosphate, and O2, whereas its 3 products are acetyl phosphate, CO2, and H2O2. This enzyme belongs to the family of oxidoreductases, specifically those acting on the aldehyde or oxo group of donor with oxygen as acceptor. This enzyme participates in pyruvate metabolism. It has 2 cofactors: FAD, and Thiamin diphosphate.
Abbr
Pyruvate oxidase (Microorganism)
Applications
This enzyme is useful for enzymatic determination of pyruvate, GOT, GPT in clinical analysis.
Appearance
Yellowish amorphous powder, lyophilized
Enzyme Commission Number
EC 1.2.3.3
Activity
GradeⅢ 1.5U/mg-solid or more
Contaminants
ATPase < 5.0×10⁻²% GOT, GPT < 5.0×10⁻²%
Molecular Weight
approx. 260 kDa
pH Stability
pH 5.7-6.5 (25°C, 20hr)
Michaelis Constant
3.4×10⁻⁴M (Pyruvate)
Thermal stability
below 45°C (pH 6.0, 15min)
Stability
Stable at-20°C for at least one year
Inhibitors
Fe⁺⁺,Zn⁺⁺,Cu⁺⁺,Ag⁺,Hg⁺⁺
Synonyms
EC 1.2.3.3; pyruvate: oxygen 2-oxidoreductase (phosphorylating); pyruvic oxidase; phosphate-dependent pyruvate oxidase
