Products

Enzymes for Research, Diagnostic and Industrial Use

Native Microorganism Pyruvate oxidase

Cat No.
DIA-215
Description
In enzymology, a pyruvate oxidase (EC 1.2.3.3) is an enzyme that catalyzes the chemical reaction: pyruvate + phosphate + O2 ↔ acetyl phosphate + CO2 + H2O2. The 3 substrates of this enzyme are pyruvate, phosphate, and O2, whereas its 3 products are acetyl phosphate, CO2, and H2O2. This enzyme belongs to the family of oxidoreductases, specifically those acting on the aldehyde or oxo group of donor with oxygen as acceptor. This enzyme participates in pyruvate metabolism. It has 2 cofactors: FAD, and Thiamin diphosphate.
Abbr
Pyruvate oxidase (Microorganism)
Alias
pyruvic oxidase
Source
Microorganism
Applications
This enzyme is useful for enzymatic determination of pyruvate, GOT, GPT in clinical analysis.
Appearance
Yellowish amorphous powder, lyophilized
Enzyme Commission Number
EC 1.2.3.3
Activity
GradeⅢ 1.5U/mg-solid or more
CAS No.
9001-96-1
Contaminants
ATPase < 5.0×10⁻²% GOT, GPT < 5.0×10⁻²%
Molecular Weight
approx. 260 kDa
Isoelectric point
4.3
pH Stability
pH 5.7-6.5 (25°C, 20hr)
Michaelis Constant
3.4×10⁻⁴M (Pyruvate)
Optimum pH
5.7
Optimum temperature
65°C
Thermal stability
below 45°C (pH 6.0, 15min)
Stability
Stable at-20°C for at least one year
Stabilizers
Sugars, FAD
Inhibitors
Fe⁺⁺,Zn⁺⁺,Cu⁺⁺,Ag⁺,Hg⁺⁺
Synonyms
EC 1.2.3.3; pyruvate: oxygen 2-oxidoreductase (phosphorylating); pyruvic oxidase; phosphate-dependent pyruvate oxidase
Download Datasheet:


“Pyruvate oxidase” Total Products Page

Inquiry
Related Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogDIA-169
EC No.EC 1.2.3.3
CAS No.9001-96-1
SourceAerococcuss viridans
CatalogDIA-289
EC No.
CAS No.9001-96-1
SourceE. coli
CatalogNATE-0613
EC No.EC 1.2.3.3
CAS No.9001-96-1
SourceMicroorganisms
CatalogEXWM-1210
ProductNamepyruvate oxidase
EC No.EC 1.2.3.3
CAS No.9001-96-1
Source
+ See More >>
Related Protocols
pyruvate oxidase -Enzymatic Assay Protocol
Online Inquiry
Name:
*Phone:
*E-mail Address:
Country:
Company/Institution:
*Products or Services Interested:
Quantity:
Project Description:
Our Products are Not Intended for Private Therapeutic Use!
*Verification Code:
Please input "enzymes"(case insensitive) as verification code.

Welcome! For price inquiries, please feel free to contact us through the form below through the form on the left side. We will get back to you as soon as possible.

Sitemap  Privacy Policy
Copyright ©2011 - 2017 Creative Enzymes.
Contact Us 45-1 Ramsey Road, Shirley, NY 11967, USA
Email: info@creative-enzymes.com
Tel: 1-631-562-8517 1-631-448-7888
Fax: 1-631-938-8127
Distributors To view the contact information for a specific location, select the desired country: