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Native Mushroom Tyrosinase


Cat No.
NATE-0726
Description
Tyrosinase is a copper-containing oxidase, which has activity for both catechols and cresol. It is responsible for browning reactions. This enzyme is reported to have two binding sites for aromatic substrates and a different binding site for oxygen-copper.
Abbr
Tyrosinase, Native (Mushroom)
Alias
Tyrosinase
Source
Mushroom
Applications
Tyrosinase activity has been assessed in a study that developed an alternative therapeutic agent for treating hyperpigmentation. Tyrosinase has also been used in a study to investigate the ocul ocutaneous albinism phenotype in the Pakistani population.
Product Overview
Tyrosinase is a copper-containing oxidase, which has activity for both catechols and cresol. It is responsible for browning reactions. The enzyme is reported to have two binding sites for aromatic substrates and a different binding site for oxygen-copper. The copper is probably Cu (I), with inactivation involving oxidation to Cu (II) ion. Tyrosinase is a copper-containing oxidase, which has activity for both catechols and cresol. It is responsible for browning reactions. This enzyme is reported to have two binding sites for aromatic substrates and a different binding site for oxygen-copper.
Form
lyophilized powder
Enzyme Commission Number
EC 1.14.18.1
Activity
> 1000 unit/mg solid
CAS No.
9002-10-2
Molecular Weight
128 kDa by sedimentation velocity diffusion; 133 kDa by light-scattering measurements, and 119.5 kDa by electrophoresis.
Isoelectric point
4.7-5
Unit Definition
One unit = ΔA280 of 0.001 per min at pH 6.5 at 25°C in 3 mL reaction mix containing L-tyrosine.
Optimum pH
6-7
Storage
−20°C
Synonyms
Tyrosinase; EC 1.14.18.1; 9002-10-2; monophenol monooxygenase; phenolase; monophenol oxidase; cresolase; monophenolase; tyrosine-dopa oxidase; monophenol monooxidase; monophenol dihydroxyphenylalanine:oxygen oxidoreductase; N-acetyl-6-hydroxytryptophan oxidase; monophenol, dihydroxy-L-phenylalanine oxygen oxidoreductase; o-diphenol:O2 oxidoreductase; phenol oxidase
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CatalogNATE-0612
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CatalogEXWM-0963
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Related Services
Related Protocols
TYROSINASE Catechol Oxidase Activity -Enzymatic Assay Protocol
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