Description
Alkaline phosphatase (ALP, ALKP, ALPase, Alk Phos) (EC 3.1.3.1) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. As the name suggests, alkaline phosphatases are most effective in an alkaline environment. It is sometimes used synonymously as basic phosphatase.
Abbr
ALP, Native (Porcine)
Applications
Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and hist ochemical detection. Alkaline phosphatase is also used to prevent DNA self ligation and for radiolabeling.
Package
Package size based on DEA units
Form
Lyophilized fromTris-HCl buffer, with magnesium chloride and zinc chloride, pH 8.0
Enzyme Commission Number
EC 3.1.3.1
Activity
100-300 DEA units/mg protein
Unit Definition
One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 9.8 at 37°C.
Warnings
Protein determined by biuret.
Synonyms
Alkaline phosphatase; ALP; ALKP; ALPase; Alk Phos; EC 3.1.3.1; Alkaline phosphomonoesterase; Glycerophosphatase; Phosphomonoesterase
