Native Pseudomonas sp. Cholesterol Esterase

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Cat No.

DIA-134

Description

Sterol esterase belongs to the family of hydrolases, specifically those acting on carboxylic ester bonds. The systematic name of this enzyme class is steryl-ester acylhydrolase. This enzyme participates in bile acid biosynthesis.

Abbr

Cholesterol Esterase (Pseudomonas sp.)

Source

Pseudomonas sp.

Applications

This enzyme is useful for enzymatic determination of total cholesterol when coupled with cholesterol oxidase in clinical analysis.

Appearance

Light brown amorphous powder, lyophilized

Enzyme Commission Number

EC 3.1.1.13

Activity

GradeIII 100U/mg-solid or more (containing approx. 40% of stabilizers)

CAS_No

9026-00-0

Contaminants

Catalase < 1.0×10⁻²%

Molecular Weight

approx. 300 kDa

Isoelectric point

5.9±0.1

pH Stability

pH 5.0-9.0 (25°C, 24hr)

Michaelis Constant

5.4×10⁻⁵M (Linoleate), 6.6×10⁻⁵M (Oleate), 3.7×10⁻⁵M (Linolenate), 1.5×10⁻⁴M (Palmitate), 1.2×10⁻⁴M (Myristate), 2.3×10⁻⁵M (Stearate)

Optimum pH

7.0-9.0

Optimum temperature

40°C

Thermal stability

below 55°C (pH 7.5, 10min)

Stability

Stable at-20°C for at least one year

Stabilizers

Mg⁺⁺, Na-cholate, bovine serum albumin

Inhibitors

Hg⁺⁺, Ag⁺, ionic detergents

Synonyms

cholesterol esterase; cholesteryl ester synthase; triterpenol esterase; cholesteryl esterase; cholesteryl ester hydrolase; sterol ester hydrolase; cholesterol ester hydrolase; cholesterase; acylcholesterol lipase; EC 3.1.1.13; Sterol esterase

"Cholesterol Esterase" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-1679	Native Candida Rugosa Cholesterol Esterase	EC 3.1.1.13	9026-00-0	Candida Rugosa	Inquiry
DIA-133	Native Schizophyllum commune Cholesterol Esterase	EC 3.1.1.13	9026-00-0	Schizophyllum commune	Inquiry
DIA-135	Native Microorganism Cholesterol Esterase	EC 3.1.1.13	9026-00-0	Microorganism	Inquiry
DIA-281	Chemically modified Pseudomonas species Cholesterol Esterase			Pseudomonas species	Inquiry

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