Description
Sterol esterase belongs to the family of hydrolases, specifically those acting on carboxylic ester bonds. The systematic name of this enzyme class is steryl-ester acylhydrolase. This enzyme participates in bile acid biosynthesis.
Abbr
Cholesterol Esterase (Pseudomonas sp.)
Applications
This enzyme is useful for enzymatic determination of total cholesterol when coupled with cholesterol oxidase in clinical analysis.
Appearance
Light brown amorphous powder, lyophilized
Enzyme Commission Number
EC 3.1.1.13
Activity
GradeIII 100U/mg-solid or more (containing approx. 40% of stabilizers)
Contaminants
Catalase < 1.0×10⁻²%
Molecular Weight
approx. 300 kDa
Isoelectric point
5.9±0.1
pH Stability
pH 5.0-9.0 (25°C, 24hr)
Michaelis Constant
5.4×10⁻⁵M (Linoleate), 6.6×10⁻⁵M (Oleate), 3.7×10⁻⁵M (Linolenate), 1.5×10⁻⁴M (Palmitate), 1.2×10⁻⁴M (Myristate), 2.3×10⁻⁵M (Stearate)
Thermal stability
below 55°C (pH 7.5, 10min)
Stability
Stable at-20°C for at least one year
Stabilizers
Mg⁺⁺, Na-cholate, bovine serum albumin
Inhibitors
Hg⁺⁺, Ag⁺, ionic detergents
Synonyms
cholesterol esterase; cholesteryl ester synthase; triterpenol esterase; cholesteryl esterase; cholesteryl ester hydrolase; sterol ester hydrolase; cholesterol ester hydrolase; cholesterase; acylcholesterol lipase; EC 3.1.1.13; Sterol esterase
