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Native Zymomonas mobilis Alcohol Dehydrogenase

Cat No.
NATE-1900
Description
Alcohol dehydrogenases (ADH) are a group of dehydrogenase enzymes that occur in many organisms and facilitate the interconversion between alcohols and aldehydes or ketones with the reduction of nicotinamide adenine dinucleotide (NAD+ to NADH). In Humans and many other animals, they serve to break down alcohols that otherwise are toxic, and they also participate in geneRation of useful aldehyde, ketone, or alcohol groups during biosynthesis of various metabolites. In yeast, plants, and many bacteria, some alcohol dehydrogenases catalyze the opposite reaction as part of fermentation to ensure a constant supply of NAD+.
Abbr
ADH, Native (Zymomonas mobilis)
Alias
ADH
Source
Zymomonas mobilis
Applications
The enzyme is useful for determination of alcohols or aldehydes.
Appearance
Lyophilized
Enzyme Commission Number
EC 1.1.1.1
CAS No.
9031-72-5
Contaminants
(as ZM-ADH activity = 100 %)
Glucose-6-phosphate dehydrogenase: < 0.10 %;
Glucokinase: < 0.02 %;
Pyruvate kinase: < 0.02 %;
NADH oxidase: < 0.01 %;
Lactate dehydrogenase: < 0.01 %.
Molecular Weight
ca. 148,000; Subunit molecular weight : ca. 37,000
pH Stability
7.0 - 9.0
Michaelis Constant
(100 mM Glycine-KOH buffer, pH 9.0, at 30 °C)
Ethanol: 110 mM;
Methanol: 350 mM;
NAD+: 0.12 mM;
Acetaldehyde: 1.66 mM;
NADH: 0.03 mM.
Specificity
Ethanol: 100 %;
Methanol: 0.05 %;
n - Propanol: 42.3 %;
n - Butanol: 0.28 %.
Unit Definition
One unit of activity is defined as the amount of ZM-ADH that forms 1 μmol of NADH per minute at 30 °C.
Optimum pH
9.5 - 10.0
Thermal stability
No detectable decrease in activity up to 40 °C.
Storage
Stable at -20 °C for at least six months.
Synonyms
aldehyde reductase; ADH; alcohol dehydrogenase (NAD); aliphatic alcohol dehydrogenase; ethanol dehydrogenase; NAD-dependent alcohol dehydrogenase; NAD-specific aromatic alcohol dehydrogenase; NADH-alcohol dehydrogenase; NADH-aldehyde dehydrogenase; primary alcohol dehydrogenase; yeast alcohol dehydrogenase; EC 1.1.1.1; 9031-72-5
Specific Activity
more than 400 U/mg protein
Reaction
Alcohol + NAD+ ←→ Aldehyde + NADH + H+
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Inquiry
Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-1786
EC No.EC 1.1.1.1
CAS No.9031-72-5
SourceE. coli
CatalogNATE-1787
EC No.EC 1.1.1.1
CAS No.9031-72-5
SourceE. coli
CatalogNATE-0034
EC No.EC 1.1.1.1
CAS No.9031-72-5
Source
CatalogNATE-0035
EC No.EC 1.1.1.1
CAS No.9031-72-5
SourceSaccharomyces cerevisiae
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Realted Service
Realted Protocols
MYO-INOSITOL-DEHYDROGENASE -Enzymatic Assay Protocol
polyol-dehydrogenase -Enzymatic Assay Protocol
L-FUCOSE DEHYDROGENASE -Enzymatic Assay Protocol
L-fucose dehydrogenase -Enzymatic Assay Protocol
l-xylulose reductase -Enzymatic Assay Protocol
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