Description
In enzymology, nitrile hydratases (NHases; EC 4.2.1.84) are mononuclear iron or non-corrinoid cobalt enzymes that catalyse the hydration of diverse nitriles to their corresponding amides: R-C≡N + H2O → R-C(O)NH2.
Abbr
Nhase, Recombinant (Sinorhizobium meliloti)
Source
Sinorhizobium meliloti 1021
Form
Supplied in 3.2 M ammonium sulphate
Enzyme Commission Number
EC 4.2.1.84
Molecular Weight
16126.0 Da (α-subunit), 24098.0 Da (β-subunit)
Purity
> 95 % as judged by SDS-PAGE
Unit Definition
One unit is defined as the amount of enzyme required to release 1 μmol of methacrylamide from methocrylonitrile (10 mM) per minute in 100 mM KH2PO4 buffer, pH 7.0, at 25°C, as measured at 224 nm.
Storage
Store at 4°C (shipped at room temperature)
Preparation Instructions
Agitate vial sufficiently to fully homogenise enzyme precipitate before use.
Synonyms
Nitrilase; 3-cyanopyridine hydratase; NHase; L-NHase; H-NHase; acrylonitrile hydratase; aliphatic nitrile hydratase; nitrile hydro-lyase; aliphatic-amide hydro-lyase (nitrile-forming)
