Description
In enzymology, a peptide-N4-(N-acetyl-beta-glucosaminyl) asparagine amidase (EC 3.5.1.52) is an enzyme that catalyzes a chemical reaction that cleaves a N4-(acetyl-beta-D-glucosaminyl)asparagine residue in which the glucosamine residue may be further glycosylated, to yield a (substituted) N-acetyl-beta-D-glucosaminylamine and a peptide containing an aspartate residue. This enzyme belongs to the family of hydrolases, specifically those acting on carbon-nitrogen bonds other than peptide bonds in linear amides.
Abbr
PNGase F, Recombinant (Elizabethkingia miricola)
Species
Elizabethkingia miricola
Applications
PNGase F can be use to cleave N-glycans attached to proteins and antibodies.
Form
20 mM Tris pH8, 50% glycerol.
Purity
>95% by SDS-PAGE gel
Concentration
50000unit/ml
Unit Definition
One unit is defined as the amount of enzyme required to removed >95% of the glycans from 10 ug of denatured RNase B in 1 hour at 37°C.
Stability
PNGase F retains >60% activity after left at room temperature for over 72 hours. Long term storage at - 20°C or below
Storage
Long term storage at -20°C or below
Synonyms
glycopeptide N-glycosidase; glycopeptidase; N-oligosaccharide glycopeptidase; N-glycanase; glycopeptidase; Jack-bean glycopeptidase; PNGase A; PNGase F; glycopeptide N-glycosidase; peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase; EC 3.5.1.52; PNGase F; 83534-39-8
