PNGase F from Elizabethkingia miricola, Recombinant

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Cat No.

NATE-1286

Description

In enzymology, a peptide-N4-(N-acetyl-beta-glucosaminyl) asparagine amidase (EC 3.5.1.52) is an enzyme that catalyzes a chemical reaction that cleaves a N4-(acetyl-beta-D-glucosaminyl)asparagine residue in which the glucosamine residue may be further glycosylated, to yield a (substituted) N-acetyl-beta-D-glucosaminylamine and a peptide containing an aspartate residue. This enzyme belongs to the family of hydrolases, specifically those acting on carbon-nitrogen bonds other than peptide bonds in linear amides.

Abbr

PNGase F, Recombinant (Elizabethkingia miricola)

Alias

PNGase F

Source

E. coli

Species

Elizabethkingia miricola

Applications

PNGase F can be use to cleave N-glycans attached to proteins and antibodies.

Form

20 mM Tris pH8, 50% glycerol.

CAS_No

83534-39-8

Purity

>95% by SDS-PAGE gel

Concentration

50000unit/ml

Unit Definition

One unit is defined as the amount of enzyme required to removed >95% of the glycans from 10 ug of denatured RNase B in 1 hour at 37°C.

Stability

PNGase F retains >60% activity after left at room temperature for over 72 hours. Long term storage at - 20°C or below

Storage

Long term storage at -20°C or below

Synonyms

glycopeptide N-glycosidase; glycopeptidase; N-oligosaccharide glycopeptidase; N-glycanase; glycopeptidase; Jack-bean glycopeptidase; PNGase A; PNGase F; glycopeptide N-glycosidase; peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase; EC 3.5.1.52; PNGase F; 83534-39-8

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Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-1941	PNGase A from Oryza sativa (rice), Recombinant	EC 3.5.1.52		Pichia pastoris	Inquiry
NATE-0600	Native Almonds Glycopeptidase A	EC 3.5.1.52	83534-39-8	Almonds	Inquiry
NATE-0601	Native Elizabethkingia meningoseptica PNGase F	EC 3.5.1.52	83534-39-8	Elizabethkingia meningoseptica	Inquiry
NATE-0602	Native Elizabethkingia miricola PNGase F	EC 3.5.1.52	83534-39-8	Elizabethkingia miricola	Inquiry

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