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Pyroglutamate Aminopeptidase from Pyrococcus furiosus, Recombinant


Cat No.
NATE-0648
Description
Pyroglutamate aminopetidase is an enzyme that digests proteins. This enzyme is specific for N-terminal pyroglutamic acids. It cleaves the N-terminal pyroglutamic acid from proteins and peptides prior to Edman degradation. The optimal temperature range is 95 to 100°C and the optimal pH range is 6.0 to 9.0.
Abbr
Pyrase, Native (Pyrococcus furiosus)
Alias
Pyrase
Source
E. coli
Species
Pyrococcus furiosus
Applications
Thermostable aminopeptidase that liberates N-terminal pyroglutamic acid from proteins and peptides prior to Edman degradation. Pyroglutamate Aminopeptidase, from Pyrococcus furiosus is a recombinant, thermostable aminopeptidase that is expressed in Escherichia coli. It is used to cleave pyroglutamic acid which allows analysis of N-terminal sequences of peptides. The enzyme from Creative Enzymes has been used for the removal of pyroglutamate (pGlu) N-terminal blocking group, under reduced conditions, prior to N-terminal sequencing of purified cassiicolin.
Product Overview
recombinant, expressed in E. coli, ~90% (SDS-PAGE)
Form
Lyophilized powder containing sodium phosphate
Enzyme Commission Number
EC 3.4.19.3
Activity
> 0.11 units/mg protein
CAS No.
9075-21-2
Molecular Weight
mol wt 24.072 kDa by amino acid sequence mol wt 28 kDa by SDS-PAGE
Unit Definition
One unit will hydrolyze 1 μmol of pyroglutamate p-nitroanilide per minute at pH 7.0 at 37°C.
Optimum pH
6.0 to 9.0
Optimum temperature
95 to 100°C
Storage
−20°C
Synonyms
pyroglutamyl-peptidase I; Pyroglutamate aminopeptidase; EC 3.4.19.3; 5-oxoprolyl-peptidase; pyrase; pyroglutamate aminopeptidase; pyroglutamyl aminopeptidase; L-pyroglutamyl peptide hydrolase; pyrrolidone-carboxyl peptidase; pyrrolidone-carboxylate peptidase; pyrrolidonyl peptidase; L-pyrrolidonecarboxylate peptidase; pyroglutamidase; pyrrolidonecarboxylyl peptidase; 9075-21-2
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Related Protocols
pyroglutamate aminopeptidase -Enzymatic Assay Protocol
L-pyroglutamate aminopeptidase -Enzymatic Assay Protocol
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