Products

Enzymes for Research, Diagnostic and Industrial Use

Pyroglutamate Aminopeptidase from Pyrococcus furiosus, Recombinant

Cat No.
NATE-0648
Description
Pyroglutamate aminopetidase is an enzyme that digests proteins. This enzyme is specific for N-terminal pyroglutamic acids. It cleaves the N-terminal pyroglutamic acid from proteins and peptides prior to Edman degradation. The optimal temperature range is 95 to 100°C and the optimal pH range is 6.0 to 9.0.
Abbr
Pyrase, Native (Pyrococcus furiosus)
Alias
Pyrase
Source
E. coli
Species
Pyrococcus furiosus
Applications
Thermostable aminopeptidase that liberates N-terminal pyroglutamic acid from proteins and peptides prior to Edman degradation. Pyroglutamate Aminopeptidase, from Pyrococcus furiosus is a recombinant, thermostable aminopeptidase that is expressed in Escherichia coli. It is used to cleave pyroglutamic acid which allows analysis of N-terminal sequences of peptides. The enzyme from Creative Enzymes has been used for the removal of pyroglutamate (pGlu) N-terminal blocking group, under reduced conditions, prior to N-terminal sequencing of purified cassiicolin.
Product Overview
recombinant, expressed in E. coli, ~90% (SDS-PAGE)
Form
Lyophilized powder containing sodium phosphate
Enzyme Commission Number
EC 3.4.19.3
Activity
> 0.11 units/mg protein
CAS No.
9075-21-2
Molecular Weight
mol wt 24.072 kDa by amino acid sequence mol wt 28 kDa by SDS-PAGE
Unit Definition
One unit will hydrolyze 1 μmol of pyroglutamate p-nitroanilide per minute at pH 7.0 at 37°C.
Optimum pH
6.0 to 9.0
Optimum temperature
95 to 100°C
Storage
−20°C
Synonyms
pyroglutamyl-peptidase I; Pyroglutamate aminopeptidase; EC 3.4.19.3; 5-oxoprolyl-peptidase; pyrase; pyroglutamate aminopeptidase; pyroglutamyl aminopeptidase; L-pyroglutamyl peptide hydrolase; pyrrolidone-carboxyl peptidase; pyrrolidone-carboxylate peptidase; pyrrolidonyl peptidase; L-pyrrolidonecarboxylate peptidase; pyroglutamidase; pyrrolidonecarboxylyl peptidase; 9075-21-2
Download Datasheet:


“Pyrase” Total Products Page

Inquiry
Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-0647
EC No.EC 3.4.19.3
CAS No.9075-21-2
SourceBacillus sp.
CatalogNATE-1256
EC No.
CAS No.
SourceThermococcus thioreducens
CatalogEXWM-4088
EC No.EC 3.4.19.3
CAS No.9075-21-2
Source
Realted Protocols
pyroglutamate aminopeptidase -Enzymatic Assay Protocol
L-pyroglutamate aminopeptidase -Enzymatic Assay Protocol
Online Inquiry
Name:
*Phone:
*E-mail Address:
Country:
Company/Institution:
*Products or Services Interested:
Quantity:
Project Description:
Our Products are Not Intended for Private Therapeutic Use!
*Verification Code:
Please input "enzymes"(case insensitive) as verification code.

Welcome! For price inquiries, please feel free to contact us through the form below through the form on the left side. We will get back to you as soon as possible.

Sitemap  Privacy Policy
Copyright ©2011 - 2017 Creative Enzymes.
Contact Us 45-1 Ramsey Road, Shirley, NY 11967, USA
Email: info@creative-enzymes.com
Tel: 1-631-562-8517 1-631-448-7888
Fax: 1-631-938-8127
Distributors To view the contact information for a specific location, select the desired country: