Reducing-end cellobiohydrolase 48A from Clostridium stercorarium, Recombinant

Cat No.

NATE-1326

Description

Cellulose 1,4-beta-cellobiosidase (reducing end) (EC 3.2.1.176, CelS, CelSS, endoglucanase SS, cellulase SS, cellobiohydrolase CelS, Cel48A) is an enzyme with systematic name 4-beta-D-glucan cellobiohydrolase (reducing end). This enzyme catalyses the following chemical reaction: Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and similar substrates, releasing cellobiose from the reducing ends of the chains. The CelS enzyme from Clostridium thermocellum is the most abundant subunit of the cellulosome formed by the organism.

Abbr

Cellobiohydrolase 48A, Recombinant (Clostridium stercorarium)

Source

E. coli

Species

Clostridium stercorarium

Form

35 mM NaHepes buffer, pH 7.5, 750 mM NaCl, 200 mM imidazol, 3.5 mM CaCl2, 0.02% sodium azide and 25% (v/v) glycerol

Enzyme Commission Number

EC 3.2.1.176

Molecular Weight

73.5 kDa

Purity

>50% by SDS-PAGE

Concentration

1 mg/mL

Specificity

Avicel and crystalline forms of cellulose

Optimum pH

5.0-6.0

Optimum temperature

70-75 °C

Storage

This enzyme is shipped at room temperature but should be stored at -20 °C.

Synonyms

Cellulose 1,4-beta-cellobiosidase (reducing end); EC 3.2.1.176; CelS; CelSS; endoglucanase SS; cellulase SS; cellobiohydrolase CelS; Cel48A; 4-beta-D-glucan cellobiohydrolase (reducing end)

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