Description
Cellulose 1,4-beta-cellobiosidase (reducing end) (EC 3.2.1.176, CelS, CelSS, endoglucanase SS, cellulase SS, cellobiohydrolase CelS, Cel48A) is an enzyme with systematic name 4-beta-D-glucan cellobiohydrolase (reducing end). This enzyme catalyses the following chemical reaction: Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and similar substrates, releasing cellobiose from the reducing ends of the chains. The CelS enzyme from Clostridium thermocellum is the most abundant subunit of the cellulosome formed by the organism.
Abbr
Cellobiohydrolase 48A, Recombinant (Clostridium stercorarium)
Species
Clostridium stercorarium
Form
35 mM NaHepes buffer, pH 7.5, 750 mM NaCl, 200 mM imidazol, 3.5 mM CaCl2, 0.02% sodium azide and 25% (v/v) glycerol
Enzyme Commission Number
EC 3.2.1.176
Molecular Weight
73.5 kDa
Specificity
Avicel and crystalline forms of cellulose
Optimum temperature
70-75 °C
Storage
This enzyme is shipped at room temperature but should be stored at -20 °C.
Synonyms
Cellulose 1,4-beta-cellobiosidase (reducing end); EC 3.2.1.176; CelS; CelSS; endoglucanase SS; cellulase SS; cellobiohydrolase CelS; Cel48A; 4-beta-D-glucan cellobiohydrolase (reducing end)
