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β-Glucosidase


Official Full Name
β-Glucosidase
Background
Beta-glucosidase is a glucosidase enzyme that acts upon β1->4 bonds linking two glucose or glucose-substituted molecules (i.e., the disaccharide cellobiose). It is one of the cellulases, enzymes involved in the decomposition of cellulose and related polysaccharides; more specifically, an exocellulase with specificity for a variety of beta-D-glycoside substrates. It catalyzes the hydrolysis of terminal non-reducing residues in beta-D-glucosides with release of glucose.
Synonyms
EC 3.2.1.21; gentiobiase; cellobiase; emulsin; elaterase; aryl-beta-glucosidase; beta-D-glucosidase; beta-glucoside glucohydrolase; arbutinase; amygdalinase; p-nitrophenyl beta-glucosidase; primeverosidase; amygdalase; linamarase; salicilinase; beta-1#6-glucosidase

Catalog
Product Name
EC No.
CAS No.
Source
Price
CatalogNATE-1942
EC No.EC 3.2.1-
CAS No.
SourceFlavobacterium ...
CatalogEXWM-3923
EC No.EC 3.2.1.6
CAS No.62213-14-3
Source
CatalogEXWM-3886
ProductNameβ-glucosidase
EC No.EC 3.2.1.21
CAS No.9001-22-3
Source
CatalogNATE-1438
EC No.EC 3.2.1.21
CAS No.9001-22-3
SourceE. coli
CatalogNATE-1437
EC No.EC 3.2.1.21
CAS No.9001-22-3
SourceE. coli
CatalogNATE-1436
EC No.EC 3.2.1.21
CAS No.9001-22-3
SourceE. coli
CatalogNATE-1435
EC No.EC 3.2.1.21
CAS No.9001-22-3
SourceE. coli
CatalogNATE-1434
EC No.EC 3.2.1.21
CAS No.9001-22-3
SourceE. coli
CatalogNATE-1433
EC No.EC 3.2.1.21
CAS No.9001-22-3
SourceE. coli
CatalogNATE-1432
EC No.EC 3.2.1.21
CAS No.9001-22-3
SourceE. coli
CatalogNATE-1431
EC No.EC 3.2.1.21
CAS No.9001-22-3
SourceE. coli
CatalogNATE-1430
EC No.EC 3.2.1.21
CAS No.9001-22-3
SourceE. coli
CatalogNATE-1429
EC No.EC 3.2.1.21
CAS No.9001-22-3
SourceE. coli
CatalogNATE-1428
EC No.EC 3.2.1.21
CAS No.9001-22-3
SourceE. coli
CatalogNATE-1234
EC No.EC 3.2.1.21
CAS No.9001-22-3
SourceStreptococcus p...
CatalogNATE-1183
EC No.EC 3.2.1.21
CAS No.9001-42-7
SourceRhizobium etli ...
CatalogNATE-1182
EC No.EC 3.2.1.21
CAS No.9001-42-7
SourceClostridium the...
CatalogNATE-1181
EC No.EC 3.2.1.21
CAS No.9001-42-7
SourceBacteroides fra...
CatalogNATE-0772
EC No.
CAS No.9001-22-3
SourceE. coli
CatalogNATE-0771
EC No.EC 3.2.1.6
CAS No.62213-14-3
SourceThermotoga neop...
CatalogNATE-0306
EC No.EC 3.2.1-
CAS No.9001-22-3
SourceAspergillus nig...
CatalogNATE-0769
EC No.EC 3.2.1.31
CAS No.9001-22-3
SourceAlmonds
CatalogNATE-0770
EC No.EC 3.2.1.6
CAS No.9001-22-3
SourceSweet almonds
CatalogDIA-195
EC No.EC 3.2.1.21
CAS No.9001-22-3
SourceSweet almond
Related Services
Related Protocols
LAMINARINASE -Enzymatic Assay Protocol
ß-GLUCOSIDASE -Enzymatic Assay Protocol
Related Reading

β-Glucosidase (β-D-Glucosidase, EC3.2.1.21), also known as β-D-glucosidase glucohydrolase, alias gentiobiase, cellobiase (cellobias, CB or β-G ) And amygdalase. It belongs to the cellulase class and is an important component of the cellulolytic enzyme system. It can hydrolyze the non-reducing β-D-glucose bond bound to the terminal and release β-D-glucose and the corresponding ligand at the same time.

Enzyme structure of β-Glucosidase. Figure 1. Enzyme structure of β-Glucosidase.

Source

In 1837, Liebig and Wohler first discovered β-glucosidase in bitter almond juice. The enzyme is widely distributed in nature, especially in plant seeds and microorganisms. It is also found in animals and fungi. The plant sources of β-glucosidase are ginseng, soybeans, etc.; there are many reports of microbial sources, such as Flavobacterium meningosepticum, Flavobacterium johnsonae, etc., eukaryotic sources.

Classifications

Catalytic mechanism

Most glucosidases are reserved glucosidases, and their catalytic mechanism usually follows the "two-step" mechanism.

Step 1: the carboxyl anion as a nucleophilic group nucleophilically attacks the anomeric carbon on the glycosidic bond, and at the same time, as another generalized acid-base pair, it catalyzes the formation of hydrogen bonds between the hydrogen on the carboxyl group and the oxygen atom on the glycosidic bond. The formation of Oxocarbenium-like transition state (Oxocarbenium-like transition state). After the bond is formed and broken, the anomeric carbon configuration of the glycosyl molecule is reversed for the first time and forms an ester bond with the nucleophilic carboxyl group to generate a glycosyl-enzyme covalent intermediate, and at the same time release a molecule of glycoside.

Step 2: The active hydroxyl hydrogen of the glycosyl acceptor molecule interacts with the dissociated generalized acid-base pair carboxyl ion, and the active hydroxyl oxygen of the acceptor molecule nucleophilically attacks the glycosyl molecule in the glycosyl-enzyme covalent intermediate The anomeric carbon once again forms an oxygen-containing carbocation-like transition state, and finally the configuration of the anomeric carbon is reversed for the second time and forms a covalent bond with the acceptor hydroxyl oxygen to complete the reaction.

Functions

Glucosidase is one of the important members in the carbohydrate metabolism pathway in organisms. β-glucosidase can participate in the metabolism of cellulose and a variety of physiological and biochemical pathways, and α-glucosidase is directly involved in the metabolic pathways of starch and glycogen. Abnormal functions of these enzymes can lead to metabolic diseases. At the same time, these enzymes are also targeted for various drugs and inhibitors to regulate the chemical metabolism of sugar in the human body.

Applications

Reference

  1. Jeng WY.; et al. Structural and functional analysis of three β-glucosidases from bacterium Clostridium cellulovorans, fungus Trichoderma reesei and termite Neotermes koshunensis. Journal of Structural Biology. 2011,173 (1): 46–56.

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