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β-N-Acetylhexosaminidase

Official Full Name
β-N-Acetylhexosaminidase
Background
This enzyme releases non-reducing terminal β1-2, β1-3, β1-4 and β1-6 linked N-acetylglucosamine from complex carbohydrates. When incubated with oligosaccharides at low concentrations (<50 mU/ml) the enzyme can differentiate between GlcNAcβ1-2Man, GlcNAcβ1-4Man and GlcNAcβ1-6Man linkages. Under such conditions, the enzyme cleaves essentially only β1-2 linked GlcNAc, with two provisos. Firstly, β1-2 GlcNAc is not hydrolyzed if the mannose to which it is substituted has a substitution at C-6. Thus, the enzyme is useful for the analysis of tri-antennary oligosaccharides. Secondly, if the β-linked mannose of the conserved pentasaccharide core is substituted with a "bisecting" GlcNAc then only the β1-2 linked GlcNAc linked to mannose on the α1-3 arm is cleaved. At higher concentrations of the enzyme, β1-4 and β1-6 linked GlcNAc may also be hydrolyzed.
Synonyms
beta-N-acetyl-D-hexosaminide; N-acetylhexosaminohydrolase; β-N-Acetylhexosaminidase; N-Acetyl-β-D-glucosaminidase, β-N-Acetylglucosaminidase

Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogEXWM-3915
EC No.EC 3.2.1.52
CAS No.9012-33-3
Source
CatalogNATE-1290
EC No.
CAS No.
SourceE. coli
CatalogNATE-1289
EC No.
CAS No.
SourceE. coli
CatalogNATE-1220
EC No.EC 3.2.1.52
CAS No.9012-33-3
SourceStreptococcus p...
CatalogNATE-1044
EC No.EC 3.2.1.52
CAS No.9012-33-3
SourceMicrobial
CatalogNATE-1043
EC No.EC 3.2.1.52
CAS No.9012-33-3
SourceProkaryote
CatalogNATE-0934
EC No.
CAS No.
SourceE.coli
CatalogNATE-0784
EC No.
CAS No.
SourceE. coli
CatalogNATE-0783
EC No.
CAS No.
SourceJack bean
CatalogNATE-0782
EC No.3.2.1.52
CAS No.9012-33-3
SourceE. coli
CatalogNATE-0781
EC No.EC 3.2.1.52
CAS No.9012-33-3
SourceHuman placenta
CatalogNATE-0780
EC No.EC 3.2.1.52
CAS No.9012-33-3
SourceCanavalia ensif...
CatalogNATE-0779
EC No.EC 3.2.1.52
CAS No.9012-33-3
SourceBovine kidney
Related Services
Related Protocols
b-n-acetylglucosaminidase -Enzymatic Assay Protocol
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