Description
Beta-mannosidase is an enzyme with system name beta-D-mannoside mannohydrolase. This enzyme catalyses the following chemical reaction:Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides. This gene encodes a member of the glycosyl hydrolase 2 family. The encoded protein localizes to the lysosome where it is the final exoglycosidase in the pathway for N-linked glycoprotein oligosaccharide catabolism. Mutations in this gene are associated with beta-mannosidosis, a lysosomal storage disease that has a wide spectrum of neurological involvement.
Abbr
β-Mannosidase, Recombinant (Cellvibrio mixtus)
Form
Supplied in 3.2 M ammonium sulphate
Enzyme Commission Number
EC 3.2.1.25
Molecular Weight
50300 Da
Purity
> 95 % as judged by SDS-PAGE
Unit Definition
One unit is defined as the amount of enzyme required to release 1μmol of p-nitrophenol per hour from p-nitrophenyl-β-mannopyranoside (1 mM in the assay) in 50 mM phosphate buffer, pH 7.0, at 37°C, containing 1 mg/ml of BSA.
Optimum pH
7 (stable from 6.5 – 7.5)
Optimum temperature
37°C (stable up to 40°C)
Storage
Store at 4°C (shipped at room temperature)
Synonyms
β-mannosidase; mannanase; mannase; β-D-mannosidase; β-mannoside mannohydrolase; exo-β-D-mannanase; EC 3.2.1.25; 9025-43-8
