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β-Acetylglucosaminidase 18A from Bacteroides thetaiotaomicron, Recombinant

Cat No.
NATE-1291
Description
β-acetylglucosaminidase 18A, is an enzyme from Bacteroides thetaiotaomicron that participates in the endohydrolysis of the diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the (Man(GlcNAc)(2))Asn-structure. One N-acetyl-D-glucosamine residue remains attached to the protein; the rest of the oligosaccharide is released intact. Recombinant BtAcp18A (GH18), purified from Escherichia coli, is a single domain family 18 Glycoside Hydrolase (GH18).
Abbr
β-acetylglucosaminidase 18A, Recombinant (Bacteroides thetaiotaomicron)
Source
E. coli
Species
Bacteroides thetaiotaomicron
Form
35 mM NaHepes buffer, pH 7.5, 750 mM NaCl, 200 mM imidazol, 3.5 mM CaCl2, 0.02% sodium azide and 25% (v/v) glycerol.
Enzyme Commission Number
EC 3.2.1.96
CAS No.
37278-88-9
Molecular Weight
50.3 kDa
Purity
>50% by SDS-PAGE
Concentration
1 mg/mL
Specificity
Mammalian high mannose N-glycans (HMNG), such as Man9GlcNAc2
Optimum pH
7.5
Optimum temperature
37 °C
Storage
This enzyme is shipped at room temperature but should be stored at -20 °C.
Synonyms
beta-N-acetyl-D-hexosaminide; N-acetylhexosaminohydrolase; β-N-Acetylhexosaminidase; N-Acetyl-β-D-glucosaminidase, β-N-Acetylglucosaminidase
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