Description
Diaphorase catalyzes the reaction of a reduced di- or tri-phosphopyridine nucleotide hydrogen donor with a hydrogen acceptor, usually a dye in the leucoform.
Abbr
Diaphorase, Native (Bacillus stearothermophilus)
Source
Bacillus stearothermophilus
Applications
The enzyme is useful for the measurement of various dehydrogenase reactions in visible spectral range.
Enzyme Commission Number
EC 1.6.99.-
Contaminants
(as Diaphorase activity = 100 %)
Adenylate kinase: < 0.01 %;
NADH oxidase: < 0.01 %.
Molecular Weight
ca. 30,000
Unit Definition
One unit of activity is defined as the amount of Di-1 that reduces 1 μmol of DCIP per minute at 30 °C.
Thermal stability
No detectable decrease in activity up to 50 °C.
Storage
Stable at -20 to 5 °C for at least one year.
Synonyms
Diaphorase 1; Di-1; EC 1.6.99 -
Specific Activity
more than 1,000 U/mg protein
Reaction
NAD(P)H + Acceptor(ox.) + H+ ←→ NAD(P)+ + Acceptor(red.)
