Products

Enzymes for Research, Diagnostic and Industrial Use

Native Bacillus stearothermophilus Diaphorase 1

Cat No.
NATE-1901
Description
Diaphorase catalyzes the reaction of a reduced di- or tri-phosphopyridine nucleotide hydrogen donor with a hydrogen acceptor, usually a dye in the leucoform.
Abbr
Diaphorase, Native (Bacillus stearothermophilus)
Alias
Diaphorase
Source
Bacillus stearothermophilus
Applications
The enzyme is useful for the measurement of various dehydrogenase reactions in visible spectral range.
Appearance
Lyophilized
Enzyme Commission Number
EC 1.6.99.-
Contaminants
(as Diaphorase activity = 100 %)
Adenylate kinase: < 0.01 %;
NADH oxidase: < 0.01 %.
Molecular Weight
ca. 30,000
pH Stability
7.5 - 9.5
Unit Definition
One unit of activity is defined as the amount of Di-1 that reduces 1 μmol of DCIP per minute at 30 °C.
Optimum pH
8
Thermal stability
No detectable decrease in activity up to 50 °C.
Storage
Stable at -20 to 5 °C for at least one year.
Synonyms
Diaphorase 1; Di-1; EC 1.6.99 -
Specific Activity
more than 1,000 U/mg protein
Reaction
NAD(P)H + Acceptor(ox.) + H+ ←→ NAD(P)+ + Acceptor(red.)
Download Datasheet:


“Diaphorase” Total Products Page

Inquiry
Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogDIA-142
EC No.EC 1.6.99.3
CAS No.9079-67-8
SourceBacillus megaterium
CatalogDIA-187
EC No.EC 1.6.99.-
CAS No.
SourceClostridium sp.
CatalogDIA-275
EC No.EC 1.6.99.-
CAS No.
SourceMicroorganism
CatalogDIA-320
EC No.EC 1.8.1.4
CAS No.
SourceE. coli
+ See More >>
Online Inquiry
Name:
*Phone:
*E-mail Address:
Country:
Company/Institution:
*Products or Services Interested:
Quantity:
Project Description:
Our Products are Not Intended for Private Therapeutic Use!
*Verification Code:
Please input "enzymes"(case insensitive) as verification code.

Welcome! For price inquiries, please feel free to contact us through the form below through the form on the left side. We will get back to you as soon as possible.

Sitemap  Privacy Policy
Copyright ©2011 - 2017 Creative Enzymes.
Contact Us 45-1 Ramsey Road, Shirley, NY 11967, USA
Email: info@creative-enzymes.com
Tel: 1-631-562-8517 1-631-448-7888
Fax: 1-631-938-8127
Distributors To view the contact information for a specific location, select the desired country: