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Native Enterobacter cloacae β-Lactamase

Cat No.
NATE-0774
Description
β--lactamase inactivates β-lactam antibiotics by breaking open the β-lactam ring.
Abbr
β-Lactamase, Native (Enterobacter cloacae)
Alias
β-lactamase
Source
Enterobacter cloacae
Applications
β-lactamase is used to inactivate β-lactam antibiotics by breaking open the β-lactam ring. β-lactamase is used to study antibiotic resistance and resistance suppression1. This product is produced from Enterobacter cloacae.
Product Overview
Protein determined by biuret
Form
Lyophilized powder containing sodium phosphate and sodium Citrate buffer salts
Enzyme Commission Number
EC 3.5.2.6
Activity
6-18 units/mg protein (using benzylpenicillin); 0.2-0.6 units/mg protein (using benzylpenicillin)
CAS No.
9073-60-3
Unit Definition
One unit will hydrolyze 1.0 μmole of benzylpenicillin per min at pH 7.0 at 25°C. This International Unit (using benzylpenicillin as substrate) is approximately equal to 600 Levy or 75 Pollock units.
Storage
2-8°C
Synonyms
β-lactamase; penicillinase; cephalosporinase; neutrapen; penicillin β-lactamase; exopenicillinase; ampicillinase; penicillin amido-β-lactamhydrolase; penicillinase I, II; β-lactamase I-III; β-lactamase A, B, C; β-lactamase AME I; cephalosporin-β-lactamase; EC 3.5.2.6; 9073-60-3
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Related Protocols
PENICILLINASE -Enzymatic Assay Protocol
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