Native Escherichia coli Alkaline Phosphatase

Cat No.

NATE-0056

Description

Alkaline phosphatase (ALP, ALKP, ALPase, Alk Phos) (EC 3.1.3.1) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. As the name suggests, alkaline phosphatases are most effective in an alkaline environment. It is sometimes used synonymously as basic phosphatase.

Abbr

ALP, Native (E. coli)

Alias

ALP; ALKP

Source

Escherichia coli

Applications

Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and hist ochemical detection. It may be used for protein labeling when high sensitivity is required.

Form

A suspension in 2.6M ammonium sulfate, pH 8.0.

Enzyme Commission Number

EC 3.1.3.1

Activity

Type I, >30 units per mg protein; Type II, >20 units per mg protein; Type III, >10 units per mg protein.

CAS No.

9001-78-9

Unit Definition

One Unit hydrolyzes 1µmole of p-nitrophenol phosphate per minute at 25°C, pH 8.0.

Storage

2-8°C

Warnings

Protein determined by biuret.

Synonyms

Alkaline phosphatase; ALP; ALKP; ALPase; Alk Phos; EC 3.1.3.1; Alkaline phosphomonoesterase; Glycerophosphatase; Phosphomonoesterase

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