Description
Alkaline phosphatase (ALP, ALKP, ALPase, Alk Phos) (EC 3.1.3.1) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. As the name suggests, alkaline phosphatases are most effective in an alkaline environment. It is sometimes used synonymously as basic phosphatase.
Abbr
ALP, Native (E. coli)
Applications
Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and hist ochemical detection. It may be used for protein labeling when high sensitivity is required.
Form
A suspension in 2.6M ammonium sulfate, pH 8.0.
Enzyme Commission Number
EC 3.1.3.1
Activity
Type I, >30 units per mg protein; Type II, >20 units per mg protein; Type III, >10 units per mg protein.
Unit Definition
One Unit hydrolyzes 1µmole of p-nitrophenol phosphate per minute at 25°C, pH 8.0.
Warnings
Protein determined by biuret.
Synonyms
Alkaline phosphatase; ALP; ALKP; ALPase; Alk Phos; EC 3.1.3.1; Alkaline phosphomonoesterase; Glycerophosphatase; Phosphomonoesterase