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Native Hexokinase (ADP-Dependent) from Thermococcus litoralis


Cat No.
NATE-1136
Description
In enzymology, a ADP-Dependent Hexokinase (EC 2.7.1.147) is an enzyme that catalyzes the chemical reaction: D‒Glucose + ADP → D‒Glucose‒6‒phosphate + AMP.
Abbr
Hexokinase (ADP-Dependent), Native (Thermococcus litoralis)
Source
Thermococcus litoralis
Applications
Useful for the enzymatic determination of 1,5 Anhydroglucitol.
Appearance
White amorphous powder, lyophilized
Enzyme Commission Number
EC 2.7.1.147
Activity
More than 25 U/mg solid
CAS No.
173585-07-4
Contaminants
NADPH oxidase < 0.01%; ATPase < 0.01%
Molecular Weight
50 kDa (gel filtration) 50 kDa (SDS‒PAGE)
Isoelectric point
4.1
pH Stability
6.5‒10.5
Michaelis Constant
Glucose 0.4 mM (at 37°C) ADP 0.057 mM (at 37°C)
Unit Definition
One unit is defined as the amount of enzyme which converts 1 μmole of D‒glucose to D‒Glucose‒6‒phosphate per minute at 37°C under the conditions specified in the assay procedure.
Optimum pH
7‒7.5
Optimum temperature
100°C
Activators
Mg2+
Thermal stability
Stable at 95°C and below
Storage
Storage at -20°C in the presence of a desiccant is recommended.
Synonyms
ADP-dependent glucokinase; ADP-specific glucokinase; ADP:D-glucose 6-phosphotransferase; EC 2.7.1.147
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Product Name
EC No.
CAS No.
Source
Price
CatalogNATE-1135
EC No.EC 2.7.1.147
CAS No.173585-07-4
SourcePyrococcus furiosus
CatalogEXWM-2977
EC No.EC 2.7.1.147
CAS No.173585-07-4
Source
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