Description
Pyroglutamate aminopetidase is an enzyme that digests proteins. This enzyme is specific for N-terminal pyroglutamic acids. It cleaves the N-terminal pyroglutamic acid from proteins and peptides prior to Edman degradation. The optimal temperature range is 95 to 100°C and the optimal pH range is 6.0 to 9.0.
Abbr
Pyrase, Native (Thermococcus thioreducens)
Source
Thermococcus thioreducens
Molecular Weight
21.5 kDa
Unit Definition
One unit will hydrolyze 1 µmole of pyroglutamyl-β-napthylamide per minute at pH 7.5 at 37 °C.
Optimum temperature
75-85°C
Thermal stability
25°C - 80°C
Preparation Instructions
Reanimate with 50µl of 50 mM potassium phosphate buffer, pH 7.5. Store at 4°C.
Synonyms
pyroglutamyl-peptidase I; Pyroglutamate aminopeptidase; EC 3.4.19.3; 5-oxoprolyl-peptidase; pyrase; pyroglutamate aminopeptidase; pyroglutamyl aminopeptidase; L-pyroglutamyl peptide hydrolase; pyrrolidone-carboxyl peptidase; pyrrolidone-carboxylate peptidase; pyrrolidonyl peptidase; L-pyrrolidonecarboxylate peptidase; pyroglutamidase; pyrrolidonecarboxylyl peptidase; 9075-21-2
