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Ribonuclease H from Escherichia coli, Recombinant

Cat No.
NATE-0657
Description
Ribonuclease H (RNase H) is a family of non-sequence-specific endonucleases that catalyze the cleavage of RNA via a hydrolytic mechanism. Members of the RNase H family can be found in nearly all organisms, from bacteria to archaea to eukaryotes. RNase H’s ribonuclease activity cleaves the 3’-O-P bond of RNA in a DNA/RNA duplex substrate to produce 3’-hydroxyl and 5‘-phosphate terminated products. In DNA replication, RNase H is responsible for removing the RNA primer, allowing completion of the newly synthesized DNA.
Abbr
RNase H, Recombinant (E. coli)
Alias
RNase H
Source
E. coli
Species
Escherichia coli
Applications
Ribonuclease H from Escherichia coli has been used in a study to assess metallobi ochemistry of the magnesium ion. Ribonuclease H has also been used in a study to investigate selective inhibitors of HIV-1 reverse transcriptase ass ociated Rnase H activity.
Package
vial of ~30 units
Form
buffered aqueous glycerol solution; Solution in 50% glycerol containing 20 mM Tris-HCl, pH 7.5, 100 mM KCl, 10 mM MgCl2, 0.1 mM EDTA, 0.1 mM DTT and 0.05 mg BSA per ml
Enzyme Commission Number
EC 3.1.4.34
Activity
1,000-4,000 units/mL
CAS No.
9050-76-4
Concentration
1 kDa-4 kDa units/mL
Unit Definition
One unit hydrolyzes 1.0 nanomole RNA in 3H-labeled poly (A) • poly (dT) to acid soluble material in 20 min at 37°C.
Storage
−20°C
Synonyms
Ribonuclease H; RNase H; EC 3.1.4.34; 9050-76-4
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