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Xylosidase 43A from Cellvibrio japonicus, Recombinant

Cat No.
NATE-1528
Description
Releases reducing sugars from birchwood xylan (X0502), also catalyzes the hydrolysis of 4-methylumbelliferyl-β-D-cellobioside and 4-methylumbelliferyl-β-D-glucopyranoside. This enzyme does not possess endo-xylanase, arabinoxylanase or β-glucanase activities. β-Xylosidase undergoes post-translation glycosylation which has been shown to be critical for its proper activity and stability. Deglycosylation altered the the optimum temperature and pH for activity and decreased its thermostability.
Abbr
Xylosidase 43A, Recombinant (Cellvibrio japonicus)
Source
E. coli
Species
Cellvibrio japonicus
Form
35 mM NaHepes buffer, pH 7.5, 750 mM NaCl, 200 mM imidazol, 3.5 mM CaCl2, 0.02% sodium azide and 25% (v/v) glycerol
Enzyme Commission Number
EC 3.2.1.37
CAS No.
9025-53-0
Molecular Weight
39.9 kDa
Purity
>90% as judged by SDS-PAGE
Concentration
1 mg/mL
Specificity
Sugar beet arabinan in an exo-fashion and 4-nitrophenyl-L-arabinofuranoside (4NPA)
Optimum pH
7
Optimum temperature
25 °C
Storage
This enzyme is shipped at room temperature but should be stored at -20 °C.
Synonyms
β-Xylosidase; EC 3.2.1.37; 9025-53-0; Xylosidase
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