Native Porcine Carboxypeptidase B

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Activity	Size	Price	Qty	Add to Cart	Sample	Inquiry
~50 U/mg protein	20 mg	$420		Add to Cart		Inquiry

Cat No.

NATE-0152

Description

Carboxypeptidase B (or peptidyl-L-lysine (-L-arginine) hydrolase) catalyzes the hydrolysis of the basic amino acids, lysine, arginine, and ornithine from the C-terminal position of polypeptides. It has been shown to be a single polypeptide of 34 kDa Da. Trypsin is capable of converting native enzyme to the active enzyme, carboxypeptidase B II in vitro. The optimum pH is found to be 9.0. The enzyme may be used for sequence analysis by successive cleavage of C-terminal basic amino acids. It can also be used as a serum marker for the diagnosis of acute pancreatitis.

Abbr

CPB1, Native (Porcine)

Alias

Source

Porcine pancreas

Species

Porcine

Applications

Carboxypeptidase B has been used in a study to develop a non-invasive pregnancy assay for use in both captive and wild polar bears. Carboxypeptidase B has been used in a study that identified new potential biomarkers of acute pancreatitis. The enzyme from Creative Enzymes has been used to develop homogeneous time-resolved fluorescence (HTRF) assay for measuring carboxypeptidase B activity in a miniaturized high-throughput screening format. It has been used to evaluate the impact of the C-terminal lysine (s) in human plasminogen binding to Bifidobacterium. The effect of treatment with carboxypeptidase B, which is a C-terminal lysine-specific endopeptidase, is measured using flow cytometry analysis.

Product Overview

Protein determined by biuret.

Form

lyophilized powder. Contains HEPES buffer salts and carbohydrate

Enzyme Commission Number

EC 3.4.17.2

CAS_No

9025-24-5

Unit Definition

One unit will hydrolyze 1.0 μmole of hippuryl-L-arginine per min at pH 7.65 at 25°C.

Storage

−20°C

Synonyms

carboxypeptidase B; protaminase; CPB1; pancreatic carboxypeptidase B; tissue carboxypeptidase B; peptidyl-L-lysine [L-arginine]hydrolase; EC 3.4.17.2; 9025-24-5

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-0151	Native Human Carboxypeptidase B	EC 3.4.17.2	9025-24-5	Human pancreas	Inquiry
NATE-0153	Carboxypeptidase-B rat, Recombinant	EC 3.4.17.2	9025-24-5	E. coli	Inquiry
NATE-0993	Carboxypeptidase-B from rat, Recombinant			Pichia pastoris	Inquiry
NATE-1147	Carboxypeptidase B from Porcine, Recombinant	EC 3.4.17.2		Porcine	Inquiry

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Our Products Cannot Be Used As Medicines Directly For Personal Use.

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