Carboxypeptidase B from Porcine, Recombinant

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Activity	Size	Price	Qty	Add to Cart	Sample	Inquiry
180 U/mg protein	10 mg	$298		Add to Cart		Inquiry

Cat No.

NATE-1147

Description

Carboxypeptidase B (or peptidyl-L-lysine (-L-arginine) hydrolase) catalyzes the hydrolysis of the basic amino acids, lysine, arginine, and ornithine from the C-terminal position of polypeptides. It has been shown to be a single polypeptide of 34 kDa Da. Trypsin is capable of converting native enzyme to the active enzyme, carboxypeptidase B II in vitro. The optimum pH is found to be 9.0. The enzyme may be used for sequence analysis by successive cleavage of C-terminal basic amino acids. It can also be used as a serum marker for the diagnosis of acute pancreatitis.

Abbr

CPB1, Recombinant (Porcine)

Alias

Source

Porcine

Appearance

White powder, lyophilized

Product Overview

Carboxypeptidase B (EC 3.4.17.2), also well known as protaminase, pancreatic procarboxy-peptidase B (PCPB), tissue carboxypeptidase B, peptidyl-L-lysine (L-arginine) hydrolaseis a highly pancreas-specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitis and pancreatic graft rejection. As the prototype for those exopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lysresidues with a preference for Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point. The deduced amino acid sequence of CPB predicts a 416-amino acid preproenzyme consisting of a 15-aasignal peptide, a 95-aa activation peptide and a 307-aa mature chain. The secreted CPB zymogen is converted to enzymatically active CPB by limited proteolysis by trypsin.

Enzyme Commission Number

EC 3.4.17.2

Activity

>180U/mg

Molecular Weight

About 35kDa (SDS-PAGE detection)

Purity

>90% (SDS-PAGE test)

Unit Definition

One unit will catalyze 1.0 μmole of Hip-L-Arg per min at pH 7.65 at 25°C.

Storage

Redissolved in 20% glycerol, 4°C, store at -20°C for long-term preservation, Avoid multiple freeze-thaw cycles.

Buffer

20mM NaAc, pH3.5

Synonyms

carboxypeptidase B; protaminase; CPB1; pancreatic carboxypeptidase B; tissue carboxypeptidase B; peptidyl-L-lysine [L-arginine]hydrolase; EC 3.4.17.2; 9025-24-5

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-0151	Native Human Carboxypeptidase B	EC 3.4.17.2	9025-24-5	Human pancreas	Inquiry
NATE-0152	Native Porcine Carboxypeptidase B	EC 3.4.17.2	9025-24-5	Porcine pancreas	Inquiry
NATE-0153	Carboxypeptidase-B rat, Recombinant	EC 3.4.17.2	9025-24-5	E. coli	Inquiry
NATE-0993	Carboxypeptidase-B from rat, Recombinant			Pichia pastoris	Inquiry

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Our Products Cannot Be Used As Medicines Directly For Personal Use.

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