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Carboxypeptidase B from Porcine, Recombinant


Cat No.
NATE-1147
Description
Carboxypeptidase B (or peptidyl-L-lysine (-L-arginine) hydrolase) catalyzes the hydrolysis of the basic amino acids, lysine, arginine, and ornithine from the C-terminal position of polypeptides. It has been shown to be a single polypeptide of 34 kDa Da. Trypsin is capable of converting native enzyme to the active enzyme, carboxypeptidase B II in vitro. The optimum pH is found to be 9.0. The enzyme may be used for sequence analysis by successive cleavage of C-terminal basic amino acids. It can also be used as a serum marker for the diagnosis of acute pancreatitis.
Abbr
CPB1, Recombinant (Porcine)
Alias
CPB1
Source
Porcine
Appearance
White powder, lyophilized
Product Overview
Carboxypeptidase B (EC 3.4.17.2), also well known as protaminase, pancreatic procarboxy-peptidase B (PCPB), tissue carboxypeptidase B, peptidyl-L-lysine (L-arginine) hydrolaseis a highly pancreas-specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitis and pancreatic graft rejection. As the prototype for those exopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lysresidues with a preference for Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point. The deduced amino acid sequence of CPB predicts a 416-amino acid preproenzyme consisting of a 15-aasignal peptide, a 95-aa activation peptide and a 307-aa mature chain. The secreted CPB zymogen is converted to enzymatically active CPB by limited proteolysis by trypsin.
Enzyme Commission Number
EC 3.4.17.2
Activity
>180U/mg
Molecular Weight
About 35kDa (SDS-PAGE detection)
Purity
>90% (SDS-PAGE test)
Unit Definition
One unit will catalyze 1.0 μmole of Hip-L-Arg per min at pH 7.65 at 25°C.
Storage
Redissolved in 20% glycerol, 4°C, store at -20°C for long-term preservation, Avoid multiple freeze-thaw cycles.
Buffer
20mM NaAc, pH3.5
Synonyms
carboxypeptidase B; protaminase; CPB1; pancreatic carboxypeptidase B; tissue carboxypeptidase B; peptidyl-L-lysine [L-arginine]hydrolase; EC 3.4.17.2; 9025-24-5
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Related Protocols
carboxypeptidase_b -Enzymatic Assay Protocol
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