Products

Enzymes for Research, Diagnostic and Industrial Use

Native Wheat germ Topoisomerase I

Cat No.
NATE-0709
Description
Topoisomerase I relaxes supercoiled DNA molecules. The enzyme initiates transient breakages and rejoins of phosphodiester bonds in superhelical turns of closed-circular DNA. Enzyme activity is independent of right-and left-handed superhelices.
Abbr
TOPO I, Native (Wheat germ)
Alias
TOPO I; ω-protein
Source
Wheat germ
Applications
Suitable for: • Analsis of degree of DNA supercoiling • Forming knots and circles in single stranded DNA • Converting complementary single stranded DNA to double stranded, circular forms • Rejoining nicks in double-stranded DNA
Product Overview
Topoisomerase I relaxes supercoiled DNA molecules. The enzyme initiates transient breakages and rejoins of phosphodiester bonds in superhelical turns of closedcircular dsDNA.
Form
buffered aqueous glycerol solution
Enzyme Commission Number
EC 5.99.1.2
CAS No.
80449-01-0
Molecular Weight
mol wt 97 kDa
Unit Definition
One unit will convert 1μg of supercoiled pGEM9Zf (−) plasmid DNA to the relaxed form in 30 min at 37°C.
Storage
−70°C
Warnings
To preserve enzyme activity, avoid multiple freeze-thaw cycles and frequent temperature variations.
Synonyms
Topoisomerase I; EC 5.99.1.2; type I DNA topoisomerase; untwisting enzyme; relaxing enzyme; nicking-closing enzyme; swivelase; ω-protein; deoxyribonucleate topoisomerase; topoisomerase; type I DNA topoisomerase; DNA topoisomerase; TOPO I
Download Datasheet:


“TOPO I” Total Products Page

Inquiry
Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-0707
EC No.EC 5.99.1.2
CAS No.80449-01-0
SourceHuman
CatalogNATE-0708
EC No.EC 5.99.1.2
CAS No.80449-01-0
SourceVaccinia virus
CatalogNATE-1339
EC No.
CAS No.
SourceE. coli
CatalogNATE-1340
EC No.
CAS No.
SourceBaculovirus-infected insect cells (Spodoptera frugiperda)
+ See More >>
Online Inquiry
Name:
*Phone:
*E-mail Address:
Country:
Company/Institution:
*Products or Services Interested:
Quantity:
Project Description:
Our Products are Not Intended for Private Therapeutic Use!
*Verification Code:
Please input "enzymes"(case insensitive) as verification code.

Welcome! For price inquiries, please feel free to contact us through the form below through the form on the left side. We will get back to you as soon as possible.

Sitemap  Privacy Policy
Copyright ©2011 - 2017 Creative Enzymes.
Contact Us 45-1 Ramsey Road, Shirley, NY 11967, USA
Email: info@creative-enzymes.com
Tel: 1-631-562-8517 1-631-448-7888
Fax: 1-631-938-8127
Distributors To view the contact information for a specific location, select the desired country: