α(2,6)-Sialyltransferase from Photobacterium damsela, Recombinant

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Cat No.

NATE-0759

Description

Sialyltransferases are enzymes that transfer sialic acid to nascent oligosaccharide. Each sialyltransferase is specific for a particular sugar substrate. Sialyltransferases add sialic acid to the terminal portions of the sialylated glycolipids (gangliosides) or to the N-or O-linked sugar chains of glycoproteins. Sialyltransferases belong to glycosyltransferase family 29 (CAZY GT_29) which use a nucleotide monophosphosugar as the donor (CMP-NeuA) instead of a nucleotide diphosphosugar. Sialyltransferase transfers Neu5Ac from CMP-Neu5Ac to the galactosyl terminus of acceptor molecules including glycoproteins, glycolipids, and oligosaccharides.

Abbr

α-Sialyltransferase, Recombinant (Photobacterium damsela)

Source

E. coli BL21

Species

Photobacterium damsela

Applications

Highly active α2-6 sialyltransferase has been used to prepare high levels of disialylated fragment crystals.

Form

Supplied as a lyophilized powder containing Tris-HCl and NaCl.

Enzyme Commission Number

EC 2.4.99.1

Activity

> 5 units/mg protein

CAS_No

9075-81-4

Molecular Weight

56.8 kDa

Isoelectric point

4.88

Unit Definition

One unit will catalyze the formation of 1 μmol Neu-5-Ac-α-2,6-LacMU from CMP-Neu-5-Ac and Lac-β−OMU per minute at 37°C at pH 8.0.

Optimum pH

7.5–10.0

Stability

Store the product at –20 °C. It remains active for at least 1 year when stored properly.

Preparation Instructions

Reconstitute the lyophilized powder with water to ~5 mg/mL. Solutions can be stored at 2–8°C for 1–2 months after reconstitution. They can also be aliquoted and frozen at –70°C or –20°C for 1 year. Multiple freeze-thaw cycles should be avoided.

Synonyms

α(2,6)-Sialyltransferase; Beta-galactoside alpha-2,6-sialyltransferase; Beta-galactosamide alpha-2,6-sialyltransferase; CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase; ST6Gal1; EC 2.4.99.1

"α-Sialyltransferase" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-1921	α-2,3/2,6-Sialyltransferase from Pasteurella multocida, recombinant	EC 2.4.99.1, 2.4.99.4, 3.2.1.-		Pasteurella multocida	Inquiry
NATE-0988	α-2,3-Sialyltransferase, Recombinant				Inquiry
NATE-1171	α-2,3-Sialyltransferase from Pasteurella multocida, Recombinant	EC 2.4.99.4	71124-51-1	E. coli BL21	Inquiry
NATE-1476	α-2,3/8-sialyltransferase from Campylobacter jejuni, Recombinant	EC 2.4.99.-		E. coli	Inquiry

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Our Products Cannot Be Used As Medicines Directly For Personal Use.

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