Galactan 1,3-β-galactosidase from Clostridium thermocellum, Recombinant

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Cat No.

NATE-1206

Description

Galactan 1,3-beta-galactosidase (EC 3.2.1.145, galactan (1->3)-beta-D-galactosidase) is an enzyme with system name galactan 3-beta-D-galactosidase. This enzyme catalyses the following chemical reaction: Hydrolysis of terminal, non-reducing beta-D-galactose residues in (1->3)-beta-D-galactopyranans. This enzyme removes not only free galactose, but also 6-glycosylated residues.

Abbr

Galactan 1,3-β-galactosidase, Recombinant (Clostridium thermocellum)

Source

Clostridium thermocellum ATCC 27405

Form

Lyophilised powder

Enzyme Commission Number

EC 3.2.1.145

Activity

20 U/mg

CAS_No

161515-48-6

Molecular Weight

37692 Da

Purity

> 95 % as judged by SDS-PAGE

Unit Definition

One unit is defined as the amount of enzyme required to release 1μmol of galactose-reducing-sugar equivalents per minute from β-1,3- galactan in phosphate buffer, pH 6.0, at 60°C.

Optimum pH

6 (stable from 3 - 10)

Optimum temperature

50°C (stable up to 55°C)

Storage

Store at -20°C (shipped at room temperature)

Preparation Instructions

Dissolve to 0.8 mg/mL in 50 mM phosphate buffer, pH 6.0, containing 20 mM NaCl and aliquot for storage at -20°C. The enzyme should be stable for 6 months when stored in this manner.

Synonyms

Exo-β-1,3-galactosidase; galactan 3-β-D-galactosidase; exo-beta-1,3-galactosidase; galactan 1,3-beta-galactosidase; galactan 3-betaD-galactosidase

"Galactan 1,3-β-galactosidase" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
EXWM-3828	galactan 1,3-β-galactosidase	EC 3.2.1.145	161515-48-6		Inquiry

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